Varioskan lux multimode microplate reader fluoroskan

After the serum thawed, the serum levels of EGF (catalog number: H031), SS (catalog number: H092), TNF-α (catalog number: H052-1), IFN-γ (catalog number: H025), IL-1β (catalog number: H002), and IL-6 (catalog number: H007-1-1) were measured by Mouse ELISA Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China). After treating the serum according to the kit instructions, the absorbance was measured at 450 nm in Varioskan LUX Multimode Microplate Reader Fluoroskan (Thermo Fisher Scientific, Waltham, MA, United States) to calculate the content.

A 10% tissue homogenate was obtained by grinding the gastric tissue in a 0.9% sodium chloride solution. After centrifugation at 10,000 rpm/min for 15 min, the supernatant of the 10% tissue homogenate was collected for analysis. The levels of SOD (catalog number: A001-1–2), GSH-Px (catalog number: A005-1–2), CAT (catalog number: A007-1-1) and MDA (catalog number: A003-1–2) in gastric tissue were analysed by using corresponding test kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China). The tissue homogenate was processed according to the kit instructions, and the absorbance was measured at the required wavelength in Varioskan LUX Multimode Microplate Reader Fluoroskan (Thermo Fisher Scientific, Waltham, MA, United States).