P1v2 proton chip

Manufactured by Thermo Fisher Scientific

The P1v2 Proton Chip is a high-performance laboratory equipment designed for advanced scientific applications. It is a critical component in various analytical and research processes. The core function of the P1v2 Proton Chip is to provide accurate and reliable data generation, processing, and analysis capabilities for researchers and scientists.

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Lab products found in correlation

Ion total rna seq kit v2, by Thermo Fisher Scientific (2 mentions) Ion pi sequencing 200 kit v2, by Thermo Fisher Scientific (1 mentions) Ion pi template ot2 200 kit v2, by Thermo Fisher Scientific (1 mentions) Onetouch 2 es station, by Thermo Fisher Scientific (1 mentions) Ion pi ion sphere particles, by Thermo Fisher Scientific (1 mentions) Onetouch 2 instrument, by Thermo Fisher Scientific (1 mentions)

2 protocols using p1v2 proton chip

Small RNA Sequencing Using Proton Platform

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The complementary DNA (cDNA) libraries for single-end sequencing were prepared using the Ion Total RNA-Seq Kit v2.0 (Life Technologies). The cDNA library was size selected by PAGE gel electrophoresis for miRNA sequencing. The cDNA libraries were then processed for proton sequencing. After diluting and mixing the samples, the mixture was processed on a OneTouch 2 instrument (Life Technologies) and enriched on a OneTouch 2 ES station (Life Technologies) to prepare the template-positive Ion PI™ Ion Sphere™ Particles (Life Technologies) according to the Ion PI™ Template OT2 200 Kit v2.0 (Life Technologies). After enrichment, the mixed template-positive Ion PI™ Ion Sphere™ Particles in the samples were loaded onto a P1v2 Proton Chip (Life Technologies) and sequenced on a proton sequencer according to the Ion PI Sequencing 200 Kit v2.0 (Life Technologies).

Wang Z.Y., Guo Z.D., Li J.M., Zhao Z.Z., Fu Y.Y., Zhang C.M., Zhang Y., Liu L.N., Qian J, & Liu L.N. (2017). Genome-Wide Search for Competing Endogenous RNAs Responsible for the Effects Induced by Ebola Virus Replication and Transcription Using a trVLP System. Frontiers in Cellular and Infection Microbiology, 7, 479.

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Single-end RNA-Seq with Ion Proton

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Complementary DNA (cDNA) libraries for single-end sequencing were prepared using the Ion Total RNA-Seq Kit, v2.0 (Life Technologies) according to the manufacturer's instructions. The cDNA library for miRNA sequencing was size-selected by means of PAGE gel electrophoresis. After enrichment, the mixed template-positive Ion PITM Ion Sphere particles were applied to the P1v2 Proton Chip (Life Technologies) and sequenced on Proton Sequencers. The EB-Seq algorithm was applied to identify differentially expressed genes after significance and false discovery rate (FDR) analysis, applying the following criteria: FDR < 0.05 (Benjamini et al. 2001) (link).

Zhang K., Zhong W., Li W.P., Chen Z.J, & Zhang C. (2017). miR-15a-5p levels correlate with poor ovarian response in human follicular fluid. Reproduction (Cambridge, England), 154(4).

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