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Anti cd69 fn50

Manufactured by BD
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Sourced in France

Anti-CD69 (FN50) is a monoclonal antibody that binds to the CD69 antigen, which is a cell surface glycoprotein expressed on activated T cells, B cells, and natural killer cells. The core function of this product is to detect and quantify CD69-expressing cells in research applications.

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Lab products found in correlation

Anti cd3 sp34 2, by BD (1 mentions) Anti cd4 l200, by BD (1 mentions) Anti cd95 dx2, by BD (1 mentions) Anti cd20 2h7, by BD (1 mentions) Anti hla dr clone l243, by BD (1 mentions) Anti cd27 m t271, by Miltenyi Biotec (1 mentions) Software, by FlowJo (1 mentions) Anti cd107a h4a3, by BD (1 mentions) Anti cd8 rpa t8, by BD (1 mentions) Anti pd l1 mih1, by BD (1 mentions) Fixable live dead stain, by Thermo Fisher Scientific (1 mentions) Anti cd19 hib19, by BD (1 mentions) Lsrii system, by BD (1 mentions) Sytox blue, by Thermo Fisher Scientific (1 mentions) Diva software, by BD (1 mentions)

3 protocols using anti cd69 fn50

1

Comprehensive Immunophenotyping of Blood Cells

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Immunophenotyping was performed on fresh blood at specified time points by flow cytometry using the following antibodies: anti-CD45 (clone D058–1283, Becton-Dickinson (BD) Le Pont-de-Claix, France), anti-CD3 (SP34–2, BD), anti-CD4 (L200, BD), anti-CD8 (BWl35/80, Miltenyi Biotec, Paris, France), anti-CD95 (DX2, BD), anti-CD28 (clone 28.2, Beckman Coulter), anti-CD69 (FN50, BD), anti-HLA-DR (clone L243, BD), anti-CD20 (2H7, BD), anti-CD27 (M-T271, Miltenyi), and anti-IgD (rabbit polyclonal, BioRad, Marnes-la-Coquette, France). Briefly, blood was incubated with the mixed antibodies for 15 min, red blood cells were lysed, and the cells were washed and fixed. A total of 105 cell events were acquired with a BD LSRII instrument and data was analysed using FlowJo software (Ashland, OR, USA).
Fovet C.M., Stimmer L., Contreras V., Horellou P., Hubert A., Seddiki N., Chapon C., Tricot S., Leroy C., Flament J., Massonneau J., Tchitchek N., 't Hart B.A., Zurawski S., Klucar P., Hantraye P., Deiva K., Zurawski G., Oh S., Le Grand R, & Serguera C. (2019). Intradermal vaccination prevents anti-MOG autoimmune encephalomyelitis in macaques. EBioMedicine, 47, 492-505.
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2

Multiparametric Immune Profiling

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Fixable Live/Dead stains (Invitrogen) were utilized according to manufacturer’s instructions prior to surface staining. Following quenching and washing, surface staining was performed at 4 °C in RPMI media +1% FBS unless otherwise indicated. Antibody clones (manufacturer) utilized in this study: anti-CD8: RPA-T8 (BD), anti-CD107a: H4A3 (BD), anti-PD-1: EH12.2H7 (eBioscience), anti-PD-L1: MIH1 (BD), anti-CD69: FN50 (BD), anti-β2M: TU99 (BD), anti-CD19: HIB19 (BD). Data were collected on an LSR II (BD) and analyzed using FlowJo.
Rupp L.J., Schumann K., Roybal K.T., Gate R.E., Ye C.J., Lim W.A, & Marson A. (2017). CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells. Scientific Reports, 7, 737.
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3

Flow Cytometry of Activated T Cells

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Stained cells were analyzed using an LSRII system (BD Biosciences). Data were analyzed with the Diva software (BD Biosciences). Cell viability was evaluated using SYTOX Blue (Life Technologies). The following antibodies were used: anti-CD3e (OKT3), anti-CD28 (CD28.2), anti-CD6(M-T605) and anti-CD69 (FN50); all from BD Biosciences.
Ruminski K., Celis-Gutierrez J., Jarmuzynski N., Maturin E., Audebert S., Malissen M., Camoin L., Voisinne G., Malissen B, & Roncagalli R. (2023). Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adaptors reveals molecular plasticity of the TCR signaling pathway. Frontiers in Immunology, 14, 1139123.
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