Silymarin, TAA, hydroxyproline, p-dimethylaminobenzaldehyde, 1,1,3,3-tetraethoxypropane, chloramines-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), glutathione (GSH), β-nicotinamide adenine dinucleotide phosphate, reduced form (β-NADPH) were purchased from Sigma (St. Louis, MO); perchloric acid was obtained from GFS Chemical Co. (Columbus, OH). All other reagents used in this study were of highest grade available commercially.
Chloramines t
Manufactured by Merck Group
Sourced in United States
Chloramines-T is a laboratory reagent used as a mild oxidizing agent and disinfectant. It has applications in various chemical and biological processes. The core function of Chloramines-T is to provide oxidizing capabilities for research and analytical purposes.
Automatically generated - may contain errors
Lab products found in correlation
Silymarin, by Merck Group (3 mentions)
Hydroxyproline, by Merck Group (3 mentions)
Ad340 plate reader, by Beckman Coulter (3 mentions)
Ehrlich s solution, by Merck Group (3 mentions)
5 5 dithiobis 2 nitrobenzoic acid dtnb glutathione gsh, by Merck Group (2 mentions)
P dimethylaminobenzaldehyde, by Merck Group (2 mentions)
β nicotinamide adenine dinucleotide phosphate, by Merck Group (2 mentions)
Zearalenone, by Merck Group (1 mentions)
Sg 60 f254, by Merck Group (1 mentions)
Spd 6a, by Shimadzu (1 mentions)
Fast green, by Merck Group (1 mentions)
Sirius red, by Merck Group (1 mentions)
Glutathione gsh reductase, by Merck Group (1 mentions)
Chloramine t, by Merck Group (1 mentions)
Pi apoptosis detection kit 1, by BD (1 mentions)
Got gpt assay kit, by Asan Pharmaceutical (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc, by BD (1 mentions)
Glutathione peroxidase, by Merck Group (1 mentions)
8 protocols using chloramines t
1
Silymarin-based Hepatoprotective Assay
2
Radiolabeling and Purification of Zearalenone
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zearalenone (ZEN), chloramines-T (Ch-T), ethanol and methanol were acquired from Sigma-Aldrich. Aluminum sheets thin layer chromatography (TLC) plates (20 × 25 cm) SG-60 F254 from Merck. All the chemical substances used in this study were of analytical or clinical grade & were directly used no more purification except else was stated. No-carrier-added (NCA) [125I] as NaI (185 MBq/50 μL) diluted in 0.04 M NaOH, pH 9–11 was supplied by the institute of isotopes, Hungary, >99 %. For radioactive measurement A well-type NaI scintillation γ-Counter model Scalar Ratemeter SR7 (Nuclear Enterprises Ltd., USA) was used. The mixture was completely purified using High Performance Liquid Chromatography HPLC column, provided with a Shimadzu model detector SpD-6A (SHIMADZU Cooperation, MD, USA) that contains LC-9A pumps, column Lichrosorb (RP-C18−250 mm × 4.6 mm, 5 μm)., rheodyne injector and UV spectrophotometer detector at 320 nm wavelength. At the optimal conditions an injection of 10 μ from the [125I] ZEN solution was injected in the HPLC. As recommended methanol: water (9:1,v/v/), was The mobile phase moving at 1.0 mL/min rate. Fractions were collected separately from a 1.0 mL volume up to 15 mL volume and counted with a γ-ray scintillation counter [17 ,18 (link)].
3
Liver Hydroxyproline Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver samples were homogenized in 6 N HCl, hydrolyzed at 100 °C for 18 h, and centrifuged at 9391xg for 5 min. The supernatant was dried in speed-vacuum, dissolved in H2O, and neutralized with 10 N NaOH. Samples were incubated in a chloramine-T solution [60 mM chloramines-T (Sigma, 857319), 20 mM citrate, 50 mM acetate, pH 6.5] for 25 min at room temperatures, and then in Ehrlich’s solution (Sigma, 038910) at 65 °C for additional 20 min. Hydroxyproline content was measured using a Beckman Coulter AD 340 Plate Reader (570 nm) and normalized to liver weight.
4
Quantifying Hepatic Collagen Content
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver paraffin sections were stained with 0.1% Sirius-red (Sigma, 365548) and 0.1% Fast green (Sigma, F7252) (in saturated picric acid). Liver samples were homogenized in 6 N HCl, hydrolyzed at 100°C for 18 h, and centrifuged at 10000 rpm for 5 min. Supernatant was dried in speed-vacuum, dissolved in H2O, and neutralized with 10 N NaOH. Samples were incubated in a chloramine-T solution (60 mM chloramines-T (Sigma, 857319), 20 mM citrate, 50 mM acetate, pH 6.5) for 25 min at room temperatures, and then in Ehrlich’s solution (Sigma, 038910) at 65°C for additional 20 min. Hydroxyproline content was measured using a Beckman Coulter AD 340 Plate Reader (570 nm) and normalized to liver weight.
5
Antioxidant and Metabolite Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TAA and other reagents, including silymarin, hydroxyproline, p-dimethylaminobenzaldehyde,1,1,3,3-tetraethoxypropane(TEP), chloramines-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), reduced glutathione, glutathione (GSH) reductase, GSH peroxidase (GSH-px), and β-nicotinamide adenine dinucleotide phosphate, reduced form (β-NADPH), were purchased from Sigma (St. Louis, MO); perchloric acid was obtained from GFS Chemical Co. (Columbus, OH). All other reagents used in this study were of highest grade available commercially.
6
Hepatic Hydroxyproline Quantification
7
Silymarin Hepatoprotective Mechanism Investigation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silymarin, hydroxyproline, p-dimethylaminobenzaldehyde,1,1, 3,3-tetraethoxypropane (TEP), chloramines-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), glutathione (GSH), β-nicotinamide adenine dinucleotide phosphate, reduced form (β-NADPH), TAA and other reagents were purchased from Sigma, St. Louis, MO, USA. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were acquired from Invitrogen (Carlsbad, CA, USA). Perchloric acid was obtained from GFS Chemical Co. (Columbus, OH, USA). A GOT-GPT assay kit was purchased from Asan Pharmaceutical (Hwaseong-si, Korea). Annexin V-FITC and PI Apoptosis Detection Kit I were acquired from BD Biosciences (San Jose, CA, USA). All other reagents used in this study were of the highest grade available commercially.
8
Oxidative Stress Measurement Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following reagents were purchased from Sigma Aldrich (St. Louis, MO, USA): 7-hydroxy-6-methoxycoumarin (scopoletin), curcumin, p-dimethylaminobenzaldehyde, 1,1,3,3-tetraethoxypropane (TEP), chloramines-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), reduced glutathione, glutathione reductase (GSH-Rd), glutathione peroxidase (GSH-Px), β-nicotinamide adenine dinucleotide phosphate (β-NADP), and β-NADPH. Perchloric acid was obtained from GFS Chemical Co. (Columbus, OH, USA), thiobarbituric acid (TBA) from Lancaster Co. (Lancashire, England, UK), and hydrogen peroxide from Junsei Chemical Co., Ltd. (Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!