Triazine

ITO coated glass sheets (resistivity of ~ 20 Ω/sqm) were procured from Shilpa Enterprises, India. Abcam provided CEA, CYFRA 21-1 antigens and monoclonal anti-IgG CEA and anti-IgG CYFRA 21-1 antibodies. Melamine, triazine, phosphate buffer saline (PBS) solution, bovine serum albumin (BSA) and all the other chemicals were purchased from Sigma Aldrich. All the experiments were carried out with ultrapure water (18.11 M Ω cm).

Aldehydes, triazine, benzene-1,3-disulfonyl chloride, 2-aminopyridine, malononitrile, chlorosulfonic acid, and solvents were purchased from Sigma-Aldrich Company. Also, the thin-layer chromatography (TLC) of the commercial plates (silica gel 60 F254), were purchased from Merck Company. FT-IR spectra were recorded in a spectrophotometer (PerkinElmer 781). To investigate the surface morphology of the catalyst FE-SEM images and EDX analyses provided by a Sigma ZEISS, Oxford Instruments Field Emission Scanning Electron Microscope. The morphology of prepared catalysts was investigated using TEM by a Philips CM 120, Netherlands and microscope with an accelerating voltage of 150 kV. X-ray diffraction was performed using a Philips X'pert MPD diffractometer with a Cu operating at a current of 100 mA and a voltage of 45 kV, with the Cu-Kα radiation (λ = 0.154056 nm) at the 2q range of 10–80 and scanning at the speed of 0.05° per minute. Thermogravimetric analysis (TGA) was carried out using Shimadzu DTG-60 instrument at 25 to 600 °C. The pore volume and pore size distribution were resulted from the desorption profiles of the isotherms using the Barrett–Joyner–Halenda (BJH) method. NMR spectra (Bruker 400 MHz) were used to confirm product structure by DMSO-d6 as a solvent on a Bruker DRX-400 spectrometer.