Cholesterol cholesterol ester detection kit

Fatostatin (4-[4-(4-methylphenyl)-1,3-thiazol-2-yl]-2-propylpyridine hydrobromide) was purchased from Chembridge Corporation and its chemical structure was described in Supplementary Fig. S1. Ribonuclease A, propidium iodide (PI), Oil Red O and Filipin III were purchased from Sigma-Aldrich. Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit I and Matrigel Basement Membrane Matrix were purchased from BD Bioscience. The Free Fatty Acid Quantification Kit and Cholesterol/Cholesterol Ester Detection Kit were obtained from Abcam. pHMGCoASyn-Luc construct was obtained from Dr. Hitoshi Shimano of Tsukuba University. pFASN-700-Luc and pFASN-700-mutSRE-Luc constructs were obtained from Dr. Timothy F. Osborne of Sanford-Burnham Medical Research Institute. pLDLR-Luc and pLDLR-mutSRE constructs were obtained from Addgene (Cambridge, MA).

The amounts of fatty acid and cholesterol were measured using Free Fatty Acid Quantification Kit and Cholesterol/Cholesterol Ester Detection Kit according to the manufacturer’s instructions (Abcam) in cells treated with vehicle or fatostatin (21 (link)).