Sodium chloride (nacl)

All solutions were prepared using deionized water obtained from a Milli-Q-MilliRho purification system (Millipore, Bedford, MA, USA). Analytical-grade chemicals were employed and used without additional purification. Potassium ferricyanide (<99%) and sodium hydroxide were obtained from Panreac (Barcelona, Spain), nickel chloride (<99%) from Êxodo Científica (Hortolândia, Brazil), and toluene (99.5%) from Synth (São Paulo, Brazil). Multi-walled carbon nanotubes (MWCNTs) (90%) were from Nanocyl^® NC7000^TM (Belgium) with an average diameter of 9.5 nm and an average length of 1.5 µm. Glucose and fructose were obtained from HenriFarma (São Paulo, Brazil), lysine was obtained from Farma Certa (Uberlandia, Brazil), urea from Dinamica^® (Indaiatuba, Brazil), sodium chloride, sodium phosphate dibasic, and sodium phosphate monobasic were obtained from Vetec (Duque de Caxias, Brazil) and calcium chloride, potassium chloride, and magnesium sulfate were obtained from Synth (Diadema, Brazil). The standard solutions of the interferents were prepared in 0.1 mol L⁻¹ NaOH, which was the selected supporting electrolyte following the procedure reported elsewhere [37 (link)].

Poly (vinyl alcohol) (98% hydrolysis degree, M_w 13,000–23,000 g/mol) and methanol came from Sigma-Aldrich^®, St. Louis, MO, USA. Poly(lactic acid) (M_w 200,000 g/mol) was a donation from Natureworks, Minnetonka, MN, USA. Dimethylsulfoxide, glutaraldehyde (aqueous solution 25% (v/v)), sodium chloride (Pro Analyses), dibasic sodium phosphate (P.A.) and chloroform were purchased from Vetec^® Química Fina, Brazil. Potassium chloride (P.A.) was procured from Isofar, Brazil. Monobasic potassium phosphate (P.A.) was from Reagen, Brazil. Anforicin B (the amphotericin micellar system) was from Cristália (Itapira, São Paulo, Brazil), and amphotericin B drug powder (AmB) was purchased from Indofine Chemical Company, Inc. (Hillsborough, NJ, USA).

Chemicals: Agar Müeller Hinton and Saboraud Dextrose Agar and Broth were purchased from HiMedia^® (Mumbai, India). Amphotericin B (AmB) was obtained from Infect Chemphar CO. (Hong Kong, China). Dimethyl sulfoxide (DMSO) 99.9% A.C.S spectrophotometric grade, Tween^® 80 and sodium phosphate dibasic were provided from Sigma-Aldrich (Saint Louis, MO, USA). The colorless nail lacquer was purchased from Ideal Cosméticos (Nova Lima, Brazil). Anhydrous monobasic potassium phosphate and glycerol came from Synth (São Paulo, Brazil). Sodium chloride and potassium chloride were provided from Vetec (Duque de Caxias, Brazil) and QEEL (São Paulo, Brazil), respectively.
Microorganisms: The four American Type Culture Collection strains of Candida spp. (Candida albicans ATCC 90028, C. glabrata ATCC 2001, C. tropicalis ATCC 13803 and C. parapsilosis ATCC 22019) and one Centraalbureau voor Schimmelcultures (C. dubliniensis CBS 7987) were donated by the culture collection of the Laboratory of Medical and Molecular Mycology from the Federal University of Rio Grande do Norte (UFRN, Natal, Brazil). The microorganisms were maintained in Saboraud Dextrose Broth containing 20% glycerol, frozen at −80 °C until the moment of the experiment. All stored fungi were cultured in Saboraud Dextrose Agar for 48 h at 37 °C before the experiments.