The cells were seeded on poly-l -lysine-coated (ZSGB-Bio) coverslips and fixed after culturing. After fixation with 4% paraformaldehyde solution at room temperature, the cells were washed with 0.01 M PBS solution three times. The cells were incubated with 5% goat serum blocking solution containing 0.3% Triton X-100 for 30 min. IF was performed in a wet box by overnight incubation at 4 °C with primary antibodies against CHN1 (1:150, Abcam), E-cadherin (1:200, Proteintech Group), and vimentin (1:300, Proteintech Group). Alexa Fluor® 597-conjugated secondary antibody (1:300, ZSGB-Bio) was used for incubation at 37 °C for 1 h after washing the cells with 0.01 M PBS. The cell nuclei were stained with DAPI (0.2 µg/mL, Sigma), and images were acquired using an inverted fluorescence microscope (Leica).
Alexa fluor 597 conjugated secondary antibody
Manufactured by ZSGB-BIO
Sourced in China
Alexa Fluor® 597-conjugated secondary antibody is a fluorescent dye-labeled secondary antibody. It is designed to detect and visualize primary antibodies in various immunoassay applications.
Automatically generated - may contain errors
2 protocols using alexa fluor 597 conjugated secondary antibody
1
Immunofluorescence Analysis of Cell Markers
2
Immunofluorescence Staining of Cell Markers
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The cells were seeded on the poly-L-lysine (ZSGB-Bio, China) coated coverslips and then xed post culturing. After xation by adopting 4% paraformaldehyde solution at room temperature, the cells were washed with 0.01M PBS solution for 3 times. The cells was added 5% goat serum blocking liquid with 0.3% Triton X-100 for 30 minutes. The IF was performed in the wet box by overnight incubation at 4 ℃ with primary antibodies against CHN1(1:150, Abcam, UK), E-cadherin (1:200, Proteintech Group, USA), and vimentin (1:300, Proteintech Group, USA), respectively. The Alexa Fluor® 597-conjugated secondary antibody (1:300, ZSGB-Bio, China) was incubated at 37 ℃ for 1 h after the 0.01M PBS washing. The cell nuclei were stained with DAPI (0.2 µg/ml, Sigma, USA) and images were acquired by inverted uorescence microscope (Leica, Germany).
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