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Anti occludin

Manufactured by Wuhan Servicebio Technology
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Anti-Occludin is a laboratory reagent used for the detection and analysis of occludin, a tight junction protein. It is a specific antibody that binds to occludin and can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression, localization, and function of occludin in biological samples.

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Lab products found in correlation

Gb12224, by Wuhan Servicebio Technology (2 mentions) Anti muc2, by Wuhan Servicebio Technology (2 mentions) Gb14110, by Wuhan Servicebio Technology (2 mentions) Anti mpo, by Wuhan Servicebio Technology (2 mentions) Gb111401, by Wuhan Servicebio Technology (2 mentions) Anti zo 1, by Wuhan Servicebio Technology (2 mentions) Gentle cell disassociation reagent, by STEMCELL (1 mentions) Anti pcna, by Wuhan Servicebio Technology (1 mentions) Clodronate liposome, by Yeasen (1 mentions) Anti f4 80, by Wuhan Servicebio Technology (1 mentions) Anti brdu, by Wuhan Servicebio Technology (1 mentions) Bhb colorimetric assay kit, by Cayman Chemical (1 mentions) Intesticult organoid growth medium, by STEMCELL (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Matrigel, by Corning (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions) Cy3 conjugated donkey anti rabbit ig, by Wuhan Servicebio Technology (1 mentions) Anti th, by Wuhan Servicebio Technology (1 mentions) Bx53 microscope, by Olympus (1 mentions)

5 protocols using anti occludin

1

Immunohistochemical and Immunofluorescence Analysis

Cited 1 time
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IHC and IF analyses were performed as described previously (49 (link)). Primary antibodies for IHC and IF were as followed: anti-MUC2 (GB14110; 1:500; Servicebio); anti-MPO (GB12224; 1:200; Servicebio); anti-Occludin (GB111401">GB111401; 1:200; Servicebio). Data were analyzed by Image J.
Ma L., Shen Q., Lyu W., lv L., Wang W., Yu M., Yang H., Tao S, & Xiao Y. (2022). Clostridium butyricum and Its Derived Extracellular Vesicles Modulate Gut Homeostasis and Ameliorate Acute Experimental Colitis. Microbiology Spectrum, 10(4), e01368-22.
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2

Immunohistochemical and Immunofluorescence Analysis

Cited 1 time
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IHC and IF analyses were performed as described previously (49 (link)). Primary antibodies for IHC and IF were as followed: anti-MUC2 (GB14110; 1:500; Servicebio); anti-MPO (GB12224; 1:200; Servicebio); anti-Occludin (GB111401">GB111401; 1:200; Servicebio). Data were analyzed by Image J.
Ma L., Shen Q., Lyu W., lv L., Wang W., Yu M., Yang H., Tao S, & Xiao Y. (2022). Clostridium butyricum and Its Derived Extracellular Vesicles Modulate Gut Homeostasis and Ameliorate Acute Experimental Colitis. Microbiology Spectrum, 10(4), e01368-22.
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3

Detailed Antibody Reagents for Intestinal Research

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Anti-HMGCS2 (ab137043), anti-p-STAT6 (ab263947), anti-STAT6 (ab217998), and anti-p-JAK2 antibodies (ab92552) were obtained from Abcam. Anti-JAK2 antibody (3230T) was purchased from Cell Signaling Technology. Anti-ACAT1 (16215-1-AP) and anti-BDH1 (15417-1-AP) were purchased from Proteintech. Anti-ZO-1 (GB111981), anti-Occludin (GB111401), anti-F4/80 (GB113373), anti-Brdu (GB12051), and anti-PCNA (GB12010) were purchased from Servicebio.
Dextran sulfate sodium (DSS) was obtained from MP Biomedicals. Recombinant mouse GM-CSF (96-315-03-20) and recombinant mouse IL-4 (96-214-14-20) were obtained from Peprotech. BHB (S1031) and AS1517499 (S8685) were purchased from Selleck.cn. Cell Counting Kit-8 (CCK8) was obtained from Dojindo Laboratories, Japan. Clodronate liposomes (40337ES08) were obtained from Yeasen. Lipopolysaccharides (297-473-0), BHB sodium (13613-65-5), vancomycin (1404-93-9), neomycin-sulfate (1405-10-3), metronidazole (443-48-1), and ampicillin (69-52-3) were obtained from Sigma-Aldrich. BHB Colorimetric Assay Kit (700190) was obtained from Cayman Chemical. BCA Protein Assay Kit (P0012S) were obtained from Beyotime Biotechnology. Gentle Cell Disassociation Reagent (07174), DMEM/F12 with 15 mM HEPES (36254), and IntestiCult organoid growth medium (06005) were purchased from StemCell Technologies. Matrigel (356231) was purchased from Corning.
Huang C., Wang J., Liu H., Huang R., Yan X., Song M., Tan G, & Zhi F. (2022). Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway. BMC Medicine, 20, 148.
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4

Brain and Gut Tissue Analysis

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The mice were euthanized, and their bodies were infused with a 4% paraformaldehyde solution after the behavioral tests. The brain and cecum were removed and soaked in a 30% sucrose solution to remove any excess water. The samples were then embedded in OCT and cut into sections that were 25 µm thick. The sections were blocked and treated with primary antibodies, followed by fluorescent or horseradish peroxidase (HPR)-labeled secondary antibodies. The antibodies used were anti-TH, anti-ZO-1, anti-occludin, and Cy3-conjugated donkey anti-rabbit Ig, which were all obtained from Servicebio (Wuhan, China).
Cai B., Zhong L., Wang Q., Xu W., Li X, & Chen T. (2023). Curcumin alleviates 1-methyl- 4-phenyl- 1,2,3,6-tetrahydropyridine- induced Parkinson’s disease in mice via modulating gut microbiota and short-chain fatty acids. Frontiers in Pharmacology, 14, 1198335.
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5

Immunohistochemical Analysis of Tight Junction Proteins

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Following deparaffinization, antigen retrieval, endogenous peroxidase inhibition and serum blocking, the sections were incubated with primary antibodies (anti-occludin, 1:700, Servicebio, Wuhan, China; anti-ZO-1, 1:200, HuaBio, Hangzhou, China) overnight at 4 °C. After washing with PBS, the sections were incubated with a horseradish peroxidase-labeled goat anti-rabbit antibody (1:200; Servicebio, Wuhan, China) at room temperature for 50 min. Diaminobenzidine (DAB) chromogenic reaction, nuclear counterstaining, dehydration and mounting were performed, and the slides were observed using an Olympus BX53 microscope (Olympus, Tokyo, Japan). For each slide, the mean optical density (MOD) of the positive staining area from five nonoverlapping, randomly selected fields was considered the expression level of occludin and ZO-1. Two independent observers analyzed the images using Image-Pro Plus 6.0 software (Media Cybernetics, Bethesda, MD, United States).
Wang J.K., Wei W., Zhao D.Y., Wang H.F., Zhang Y.L., Lei J.P, & Yao S.K. (2022). Intestinal mucosal barrier in functional constipation: Dose it change?. World Journal of Clinical Cases, 10(19), 6385-6398.
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