Bay43 9006

Manufactured by Cayman Chemical

Sourced in United States

Bay43-9006 is a chemical compound used in research applications. It is a small molecule inhibitor. The core function of Bay43-9006 is to inhibit the activity of specific proteins or enzymes. No further details on intended use or application are provided.

Automatically generated - may contain errors

Lab products found in correlation

Rnaimax, by Thermo Fisher Scientific (2 mentions) Retro 2, by Merck Group (1 mentions) Pd98059, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Cell Signaling Technology (1 mentions) U0126, by Cell Signaling Technology (1 mentions) Morphine, by Merck Group (1 mentions) Go6983, by Merck Group (1 mentions) U73122, by Merck Group (1 mentions) Wortmannin, by Merck Group (1 mentions) D phe cys tyr d trp arg thr pen thr nh2 ctap, by Bio-Techne (1 mentions) Cid755673, by Bio-Techne (1 mentions) Damgo, by Bio-Techne (1 mentions) U0126, by InvivoGen (1 mentions) Ly294002, by Merck Group (1 mentions)

2 protocols using bay43 9006

Inhibiting EGFR and ERK1/2 Signaling Pathways

Cited 1 time

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Chemical inhibitors of MEK, U0126 and PD98059 (Cell Signaling Technology), were used at concentrations of 10 and 50 μM, respectively. Bay43-9006 (Cayman Chemical), a chemical inhibitor of Raf, was used at 15 μM. Retro2 (Sigma–Aldrich), a retrograde trafficking inhibitor, was used at a concentration of 100 μM (Nelson et al., 2013 (link)). All chemical inhibitors were reconstituted in DMSO (Cell Signaling Technology), which served as a volume-specific vehicle control. EGFR and ERK1/2 siRNAs (Cell Signaling Technology) were transfected into SVG-A cells with RNAiMax (Thermo Fisher) at 10 pmol per well per manufacturer’s instructions. Successful transfections of the siRNAs were confirmed using BLOCK-iT Red (Thermo Fisher) (DuShane et al., 2018 (link)).

DuShane J.K., Wilczek M.P., Crocker M.A, & Maginnis M.S. (2019). High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection. Frontiers in Microbiology, 10, 783.

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Pharmacological Modulation of GPCR Signaling

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The MOR selective agonist DAMGO (Tocris Bioscience, Ellisville, MO, USA), The MOR selective antagonist D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2 (CTAP) (Tocris Bioscience), and morphine (Sigma-Aldrich, St. Louis, MO, USA) were suspended in sterile serum-free RPMI-1640 medium, and stored at −80°C prior to use. H-89 (InvivoGen, San Diego, CA, USA), LY294002 (Sigma-Aldrich, St. Louis, MO, USA), Wortmannin (Sigma-Aldrich), U0126 (InvivoGen), CID755673 (Tocris Bioscience), U73122 (Sigma-Aldrich), Bay 43-9006 (Cayman, Ann Arbor, MI, USA), farnesylthiosalicytic acid (FTS) (Cayman), and Go 6983 (Sigma-Aldrich), were suspended in DMSO and stored at −20°C until use.

Chang J.K., Cornwell W.D, & Rogers T.J. (2021). The μ-Opioid Receptor Induces miR-21 Expression and is ERK/PKCμ-Dependent. Journal of neuroimmunology, 356, 577585.

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