5 iodotubercidin

Adenosine (Ado), AMP and CPA (direct A₁R agonist) were purchased from Sigma; hPAP was graciously provided by Dr. Mark Zylka (University of North Carolina). 5’-iodotubercidin (ITU; adenosine kinase inhibitor) was purchased from Enzo Life Sciences (Farmingdale, NY). Adenosine (Ado), AMP and CPA were dissolved in 0.9% saline at pH 7.4. AMP was delivered at a concentration of 200 nmol/10 µl (Sowa et al., 2010b (link)); Ado and CPA were delivered at a dose of 10 nmol/10 µl (DeLander and Hopkins, 1987 (link), Lima et al., 2010 (link)); hPAP was delivered at a dose of 250 mU/10 µL (Zylka et al., 2008 (link)). ITU was co-administered with Ado or AMP at a concentration of 5 nmol/10 µL (Sowa et al., 2010c (link)). Intrathecal injections (hPAP, AMP + ITU, Ado + ITU, CPA) were performed using a 28 gauge, 8 mm insulin syringe (BD Biosciences, San Jose, CA) using the direct lumbar puncture method between L5-L6 (Fairbanks, 2003 (link)). Intraplantar (i.pl) injections (AMP + ITU, Ado + ITU, CPA) were performed using a 31 gauge, ½” insulin syringe (BD Biosciences) between the distal volar footpads of the right hind paw (Hurt and Zylka, 2012 (link)).

pBabe-NF2 was obtained from Addgene. Anti-Fasn siRNA (M-040091-01-0005), anti-Acaca siRNA (M-063938-01-0005), anti-Mtor (M-065427-00-0005), anti-Rptor (M-058754-01-0005), anti-Rictor (M-064598-01-0005) and non-silencing (D-001206-13-05) siRNA were purchased from Dharmacon. Individual siRNAs against Mtor (SASI_Mm01-00164496 and -00164492), Rictor (-00137732 and -00137730), Rptor (-00055298 and -00334580), Fasn (-00177858 and -00177854), Acaca (-0011590 and -00115905), and Mlycd (-00028572 and -00028576) were purchased from Sigma-Aldrich. Anti-Merlin antibodies were purchased from Abcam (#ab88957). Lipid synthesis and metabolism antibody kit (includes anti-Fasn, -phospho ACC, -ACC, -Lipin1, -ACLY, -phospho ACLY, -ACSL1, and -ACECS1 antibodies), and anti-Casp3 antibodies were purchased from Cell Signaling Technology. Anti-SREBP1 antibodies were purchased from Santa Cruz Biotechnology. Anti-GAPDH antibodies were purchased from EMD-Millipore.
Cerulenin, C75, luteolin, 5-(tetradecyloxy)-2-furoic acid (TOFA) and 5-iodotubercidin were purchased from Enzo Life Sciences. GSK2194069, dimethylsulfoxyde (DMSO), staurosporin, sodium palmitate, 70% perchloric acid, ammonium formate, acetonitrile, acetyl-coenzyme A lithium salt, malonyl coenzyme A lithium salt, propionyl-coenzyme A lithium salt, and poly-L-lysine were purchased from Sigma-Aldrich.