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Polyclonal rabbit anti furin convertase antibody pa1 062

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Polyclonal rabbit anti-furin convertase antibody (PA1-062) is a laboratory reagent used for the detection and analysis of the furin convertase enzyme in biological samples. The antibody is produced in rabbits and is designed to specifically recognize and bind to the furin convertase protein.

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2 protocols using polyclonal rabbit anti furin convertase antibody pa1 062

1

Investigating TGF-β Signaling Pathways

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A polyclonal rabbit anti-furin convertase antibody (PA1-062; diluted at 1:1000; RRID: AB_2105077) was obtained from Thermo Fisher Scientific (Beverly, MA), and a monoclonal mouse anti-α-Tubulin antibody (sc-23948; diluted at 1:2000; RRID: AB_2493181) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A goat anti-human GDNF antibody (AF-212-NA; diluted at 1:1000; RRID: AB_2111398) was obtained from R&D Systems (Minneapolis, MN). Polyclonal rabbit anti-SMAD2 (#3103; diluted at 1:1000; RRID: AB_490816), anti-SMAD3 (#9523; diluted at 1:1000; RRID: AB_2193182), and anti-SMAD4 (#9515; diluted at 1:2000; RRID: AB_2193344) antibodies were obtained from Cell Signaling Technology (Beverly, MA). Mouse myeloma cell-derived recombinant human TGF-β1 was obtained from R&D Systems (Minneapolis, MN). Furin inhibitor II (SCP0148) and SB431542 were obtained from Sigma Aldrich.
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2

Antibody and Reagent Validation for BMP Signaling

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Polyclonal rabbit antifurin convertase antibody (PA1-062; diluted 1:1000) was obtained from Thermo Scientific. Polyclonal rabbit anti-SMAD1/5/8 (N-18; sc-6031-R; diluted 1:1000) and polyclonal goat antiactin antibodies (C-11; sc-1615; diluted 1:1000) were obtained from Santa Cruz Biotechnology. Polyclonal rabbit anti-SMAD4 (9515; diluted 1:1000) and anti-phospho-SMAD1 (Ser 463/465 )/SMAD5 (Ser 463/465 )/ SMAD8 (Ser 426/428 ) (9511; diluted 1:1000) antibodies were obtained from Cell Signaling Technology. Horseradish peroxidase-conjugated goat antirabbit and donkey antigoat IgG were obtained from Bio-Rad Laboratories and Santa Cruz Biotechnology, respectively. Recombinant human BMP4, recombinant human (rh) BMP7, rh activin A and dorsomorphin dihydrochloride (dorsomorphin) were obtained from R&D Systems. Furin inhibitor I, Decanoyl-Arg-Val-Lys-Arg-chloromethylketone was purchased from EMD Millipore.
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