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Barnstead nanopure purification system

Manufactured by Thermo Fisher Scientific
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The Barnstead NANOpure purification system is a water purification device designed to produce ultrapure Type I reagent-grade water. The system utilizes a multi-stage purification process, including reverse osmosis, electrodeionization, and UV sterilization, to remove impurities and deliver consistently high-quality water for a variety of laboratory applications.

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Lab products found in correlation

Crc 25r dose calibrator, by Mirion Technologies (3 mentions) Nd 1000 spectrophotometer, by Thermo Fisher Scientific (3 mentions) Glass microfiber chromatography paper, by Agilent Technologies (2 mentions) Ph indicator paper, by Merck Group (2 mentions) P scn bn dtpa, by Macrocyclics (2 mentions) P scn bn dfo, by Macrocyclics (1 mentions) Cyclone plus phosphor imager, by PerkinElmer (1 mentions) 2480 wizard2, by PerkinElmer (1 mentions) 1470 wizard gamma counter, by PerkinElmer (1 mentions) Oligonucleotide, by Integrated DNA Technologies (1 mentions)

6 protocols using barnstead nanopure purification system

1

Radiolabeling Reagents Characterization

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All reagents were purchased from Sigma-Aldrich unless otherwise stated and were used without further purification. The chelating agents p-SCN-Bn-DFO and p-SCN-Bn-DTPA were purchased from Macrocyclics Inc. (Dallas, TX). Water was deionised using a Barnstead NANOpure purification system (Thermo Scientific) and had a resistance of >18.2 MΩ cm−1 at 25 °C. Protein concentration measurements were made on a ND-1000 spectrophotometer (NanoDrop Technologies, Inc.). Instant thin-layer chromatography (iTLC) was performed on glass microfiber chromatography paper (Agilent Technologies) and strips were analysed with a Bioscan AR-2000 radio-TLC scanner (Eckert & Ziegler). pH was determined using pH indicator paper (Merck Millipore). Radioactivity measurements were made using a CRC-25R dose calibrator (Capintec, Inc.).
Knight J.C., Mosley M.J., Kersemans V., Dias G.M., Allen P.D., Smart S, & Cornelissen B. (2019). Dual-isotope imaging allows in vivo immunohistochemistry using radiolabelled antibodies in tumours. Nuclear Medicine and Biology, 70, 14-22.
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2

Radiolabeling and Characterization Protocol

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All reagents were purchased from Sigma-Aldrich unless otherwise stated and were used without further purification. The chelating agent p-SCN-Bn-DTPA was purchased from Macrocyclics Inc. (Dallas, TX). Water was deionised using a Barnstead NANOpure purification system (Thermo Scientific) and had a resistance of > 18.2 MΩ cm−1 at 25 °C. Protein concentration measurements were made on a ND-1000 spectrophotometer (NanoDrop Technologies, Inc.). Instant thin-layer chromatography (iTLC) was performed on glass microfiber chromatography paper (Agilent Technologies), and strips were analysed with either a Bioscan AR-2000 radio-TLC scanner (Eckert & Ziegler) or a Cyclone Plus Phosphor Imager (PerkinElmer). Radioactivity measurements were determined using a CRC®-25R dose calibrator (Capintec, Inc.).
Torres J.B., Knight J.C., Mosley M.J., Kersemans V., Koustoulidou S., Allen D., Kinchesh P., Smart S, & Cornelissen B. (2017). Imaging of Claudin-4 in Pancreatic Ductal Adenocarcinoma Using a Radiolabelled Anti-Claudin-4 Monoclonal Antibody. Molecular Imaging and Biology, 20(2), 292-299.
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3

Radioactive Protein Concentration Measurements

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All reagents were purchased from Sigma-Aldrich
unless otherwise stated and were used without further purification.
Water was deionized using a Barnstead NANOpure purification system
(Thermo Scientific) and had a resistance of >18.2 MΩ cm–1 at 25 °C. Protein concentration measurements
were made on a ND-1000 spectrophotometer (NanoDrop Technologies, Inc.).
pH was determined using pH indicator paper (Merck Millipore). Radioactivity
measurements were made using a CRC-25R dose calibrator (Capintec,
Inc.) and a 2480 WIZARD2 (link) or 1470 WIZARD
gamma counter (PerkinElmer).
Knight J.C., Mosley M., Uyeda H.T., Cong M., Fan F., Faulkner S, & Cornelissen B. (2017). In Vivo Pretargeted Imaging of HER2 and TAG-72 Expression Using the HaloTag Enzyme. Molecular Pharmaceutics, 14(7), 2307-2313.
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4

Nitric Acid Purification and Trace Metal Analysis

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Nitric acid was previously purified by sub-boiling distillation using a Savillex DST 1000 (Shelton, GA, USA). The Type I water (resistivity 18.2 MΩ cm−1) was obtained by a Thermo Scientific Barnstead Nanopure purification system. The solutions were prepared daily, and all analyses were performed in the Laboratory of Analytical Instrumentation at the Federal University of Jequitinhonha and Mucuri Valley (UFVJM, Mucuri Campus), a Class 1000 clean room. Quality control for the determination of metals was carried out by the analysis of standard reference materials. There were no statistical differences between the concentration values obtained for the reference materials, and the “target values” at 95% confidence intervals using the t-test.
Santos A.P., Silva L.Z., Freire B.M., da Silva Faria M.C., Batista B.L., Rocha B.A., Barbosa F J.r, & Rodrigues J.L. (2023). Artisanal Gem Mining in Brazil: A Source of Genotoxicity and Exposure to Toxic Elements. International Journal of Environmental Research and Public Health, 20(3), 2510.
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5

Chemical and Solvent Usage in Research

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Unless otherwise noted, the chemicals and solvents used were of analytical grade and were used as received from commercial sources. Water (dd-H2O) used as reaction solvent and in biological procedures was deionized using a Barnstead NANOpure purification system (ThermoFisher, Waltham, MA).
Obermeyer A.C., Capehart S.L., Jarman J.B, & Francis M.B. (2014). Multivalent Viral Capsids with Internal Cargo for Fibrin Imaging. PLoS ONE, 9(6), e100678.
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6

Site-Specific Protein Modification Protocols

Cited 1 time
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Unless otherwise noted, all chemicals
and solvents were of analytical grade and received from commercial
sources. Water (dd-H2O) used in biological procedures and
as a reaction solvent was deionized using a Barnstead NANOpure purification
system (ThermoFisher, Waltham, MA). Oligonucleotides were purchased
from Integrated DNA Technologies (Coralville, IA) and MilliporeSigma
(Burlington, MA). The pDule-para-aminoPhe (Addgene
#85502),36 (link) pDule-3-nitroTyrosine (Addgene
#85498),50 (link) and pBad-sfGFP 150TAG (Addgene
#85483)51 (link) plasmids were gifts from Ryan
Mehl. Amicon Ultra MWCO centrifugal concentrators were obtained from
MilliporeSigma (St. Louis, MO).
Bischoff A.J., Hamerlynck L.M., Li A.J., Roberts T.D., Ginsberg N.S, & Francis M.B. (2023). Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein–Protein Conjugation Strategy. Journal of the American Chemical Society, 145(29), 15827-15837.
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