Ki67 fitc staining set

Manufactured by BD

The Ki67-FITC staining set is a laboratory equipment product designed for the detection and quantification of Ki67 protein expression in cells. Ki67 is a marker of cellular proliferation, and this set provides the necessary reagents and protocols for performing Ki67 staining using flow cytometry or immunohistochemistry techniques.

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Lab products found in correlation

Foxp3 apc staining kit, by Thermo Fisher Scientific (2 mentions) Cell proliferation dye efluor 450, by Thermo Fisher Scientific (1 mentions) Retronectin, by Takara Bio (1 mentions) Anti cd4 clone gk1, by BioXCell (1 mentions) Anti cd8 clone 2, by BioXCell (1 mentions) Ter119, by BioLegend (1 mentions) Cd24 m1 69, by BioLegend (1 mentions) Rb6 8c5, by BioLegend (1 mentions) Mouse ccr2 apc conjugated antibody, by R&D Systems (1 mentions) 5 azacytidine, by Selleck Chemicals (1 mentions) Cx3cr1 sa011f11, by BioLegend (1 mentions) Ra3 6b2, by BioLegend (1 mentions)

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Ki67-FITC (38 citations) Ki67 staining (2 citations)

3 protocols using ki67 fitc staining set

Flow Cytometry Analysis of Immune Cells

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The following fluorochrome-conjugated antibodies were used for flow cytometry: anti-mouse CD44-FITC (IM7), CD11C-APC (N418), IFNγ-APC (XMG1.2), B220-PE (RA3-6B2), CD19-FITC (1D3), CD62L-PE (MEL-14), CD8-PE (53–6.7), CD40L-PE (MR1), TNFα-PE (MP6-XT22), CD4-APC/Cy7 (RM4-5) and control IgG mAbs were purchased from Biolegend. Ki67-FITC staining set was purchased from BD. Foxp3-APC staining kit was purchased from eBiosciences. Cell Proliferation Dye eFluor™ 450 were purchased from Invitrogen. Cyclophosphamide (CTX) was from MP Biomedicals and was intraperitoneally injected to mice at 150 mg/kg. Retronectin was from Takara and was used for coating of non-treated plates (Corning) at 12 μg/ml. For T cell depletion, anti-CD4 (clone GK1.5) and anti-CD8 (clone 2.43) antibodies, both from BioXCell, were injected i.p. at 100 μg each per mouse 2 days before CTX and were administered once a week thereafter for a total of 4 injections.

Kuczma M.P., Ding Z.C., Li T., Habtetsion T., Chen T., Hao Z., Bryan L., Singh N., Kochenderfer J.N, & Zhou G. (2017). The impact of antibiotic usage on the efficacy of chemoimmunotherapy is contingent on the source of tumor-reactive T cells. Oncotarget, 8(67), 111931-111942.

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Multiparametric Flow Cytometry Analysis

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The following fluorochrome-conjugated antibodies were used for flow cytometry: anti-mouse CD11b-FITC (M1/70), CD44-FITC (IM7), CD11C-APC (N418), IFNγ-APC (XMG1.2), B220-PE (RA3-6B2), CD62L-PE (MEL-14), CD8-PE (53–6.7), CD25-PE (PC61.5), CD40L-PE (MR1), F4/80-PE (Cl:A3-1), Gr1-PE (RB6-8C5), TNFα-PE (MP6-XT22), Ly6C-PE/Cy7 (HK1.4), PD1-PE (RMP1-30), Ly6G-APC/Cy7 (1A8), CD4-APC/Cy7 (RM4-5) and control IgG mAbs were purchased from Biolegend. Granzyme B-Alexa Fluor 647 (GB11), Thy1.1-perCP (OX-7) and Ki67-FITC staining set were purchased from BD. PDCA1-PE (eBio927) and Foxp3-APC staining kit was purchased from eBiosciences. Chloromethyfluorescein diacetate succinimidyl ester (CFSE) was purchased from Invitrogen. Chemotherapeutic agents were all purchased from Sigma-Aldrich unless otherwise specified. Human granulocyte colony-stimulating factor (G-CSF, Neupogen®) was purchased from Amgen.

Lu X., Ding Z.C., Cao Y., Liu C., Habtetsion T., Yu M., Lemos H., Salman H., Xu H., Mellor A.L, & Zhou G. (2015). Alkylating agent melphalan augments the efficacy of adoptive immunotherapy using tumor-specific CD4+ T cells. Journal of immunology (Baltimore, Md. : 1950), 194(4), 2011-2021.

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Multicolor Flow Cytometry for Immune Cell Analysis

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The following anti-mouse antibodies, including Lineage cocktail (17A2; RB6-8C5, RA3-6B2; Ter-119; M1/70); CD117 (cKit, 2B8), Ly-6A/E (Sca-1, D7), CD3 (145-2C11), CD19 (6D5), B220 (RA3-6B2), Ly-6C (HK1.4), Ly-6G (1A8), CD45.1 (A20), CD24 (M1/69), and CX3CR1 (SA011F11) were purchased from Biolegend. Ki-67-FITC staining set was purchased from BD Biosciences. Mouse CCR2 APC-conjugated antibody was purchased from R&D Systems. Anti-myeloperoxidase (MPO) antibody (2D4) FITC was purchased from Abcam. Intracellular fixation and permeabilization solution kit was purchased from BD. Violet cell proliferation kit were purchased from Thermo Fisher Scientific. Cyclophosphamide monohydrate (CTX) was purchased from Tokyo Chemical Industry. 5-azacytidine was purchased from Selleckchem. Anti-mouse TNFa (XT3.11) mAb was purchased from Bio X Cell. Mouse IL6 neutralization mAb was purchased from Biolegend.

Ding Z.C., Aboelella N.S., Bryan L., Shi H, & Zhou G. (2021). The Monocytes That Repopulate in Mice After Cyclophosphamide Treatment Acquire a Neutrophil Precursor Gene Signature and Immunosuppressive Activity. Frontiers in Immunology, 11, 594540.

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