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Human cd45

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Human CD45 is a cell surface glycoprotein that is expressed on all leukocytes. It functions as a protein tyrosine phosphatase and is involved in the regulation of cell growth and differentiation.

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Lab products found in correlation

Anti human cd90, by BD (2 mentions) Cd105, by Immunostep (2 mentions) Cd146, by BD (2 mentions) Anti mouse cd45 30 f11, by BD (1 mentions) Foxp3 staining kit, by BioLegend (1 mentions) Hcd45 microbeads, by Miltenyi Biotec (1 mentions) Cd38 hit2, by BD (1 mentions) Facscalibur apparatus, by BD (1 mentions) Facscalibur, by BD (1 mentions) Perforin, by BD (1 mentions) Tim 3, by BD (1 mentions) Lag 3, by BD (1 mentions) Cd107a, by BD (1 mentions) Tigit, by Thermo Fisher Scientific (1 mentions) Anti cd3, by R&D Systems (1 mentions) Nkg2d, by BD (1 mentions) Granzyme b, by BD (1 mentions) Mouse cd45, by BD (1 mentions) Nkg2a, by Miltenyi Biotec (1 mentions) Tnf α, by BD (1 mentions)

6 protocols using human cd45

1

Comprehensive Immunophenotyping by Flow Cytometry

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Flow cytometry was performed with LSR II (BD Biosciences) using various combinations of the following mAbs: anti-human CD45 (2D1), CD19 (HIB 19), CD3 (UCHT1), CD4 (RPA-T4), CD8 (SK1), CD33 (WM53), CCR7 (G043H7), CD45RA (HI100), CD31 (WM59), CD127 (A019D5), CD25 (M-A251), CD235a (HI264); anti-mouse CD45 (30-F11), and TER119 (TER-119); and isotype control mAbs (purchased from BD Biosciences PharMingen or Biolegend). Intracellular FoxP3 staining was performed with FoxP3 Staining Kit (Biolegend) according to the manufacturer's instructions.
Yoshihara S., Li Y., Xia J., Danzl N., Sykes M, & Yang Y.G. (2019). Posttransplant Hemophagocytic Lymphohistiocytosis Driven by Myeloid Cytokines and Vicious Cycles of T-Cell and Macrophage Activation in Humanized Mice. Frontiers in Immunology, 10, 186.
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2

Engraftment and Multilineage Reconstitution in NSG Mice

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Eight-week-old NSG mice were sublethally irradiated (300 rad) and transplanted with 1 × 106 unedited and edited CD34+ cells. Donor chimerism was assessed every 4 weeks as the frequency of human cells in total white blood cells by flow cytometry after red blood cell lysis and staining with human CD45 (BD Biosciences). Upon sacrifice, 16 or 20 weeks after transplantation, the chimeric bone marrow was harvested and assayed for level of engraftment and multilineage reconstitution after staining with the following antibodies: CD45 (2D1), CD19 (HIB19), CD33 (P67.6), CD34 (8G12), and CD38 (HIT2) (BD Biosciences) and CD41 (VIPL3) (Invitrogen). For the secondary transplantations, bone marrow cells were enriched for human CD45+ cells by immunomagnetic separation (hCD45 microbeads, Miltenyi Biotec), and the same number of human cells (5 × 106) was injected into each secondary recipient. Secondary recipients were sacrificed 10 weeks after transplantation. All in vivo experiments were conducted with approval from the institutional animal care and use committee.
Psatha N., Reik A., Phelps S., Zhou Y., Dalas D., Yannaki E., Levasseur D.N., Urnov F.D., Holmes M.C, & Papayannopoulou T. (2018). Disruption of the BCL11A Erythroid Enhancer Reactivates Fetal Hemoglobin in Erythroid Cells of Patients with β-Thalassemia Major. Molecular Therapy. Methods & Clinical Development, 10, 313-326.
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3

Engraftment of Transduced TEX Cells

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Equal numbers of TEX cells (2 × 10 5 ) transduced with shRNA in lentiviral vectors targeting NLN or a control sequence were injected into the right femur of sublethally irradiated NOD-SCID-GF mice expressing human IL-3, GM-CSF, and steel factor (41) . Five weeks after injection, mice were euthanized, and the percentage of human CD45 + (BD Biosciences) was enumerated in TEX cells by flow cytometry.
Mirali S., Botham A., Voisin V., Xu C., St-Germain J., Sharon D., Hoff F.W., Qiu Y., Hurren R., Gronda M., Jitkova Y., Nachmias B., MacLean N., Wang X., Arruda A., Minden M.D., Horton T.M., Kornblau S.M., Chan S.M., Bader G.D., Raught B, & Schimmer A.D. (2020). The mitochondrial peptidase, neurolysin, regulates respiratory chain supercomplex formation and is necessary for AML viability. Science translational medicine, 12(538).
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4

Mesenchymal Stem Cell Immunophenotyping

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Isolated stromal cells were trypsinized and centrifuged.
The cell pellet was resuspended in PBS supplemented with
5% FBS and incubated with monoclonal antibodies for 30
minutes at 4°C in the dark. Human CD45 (BD Bioscience,
USA) and CD34 (IMMUNOSTEP, Spain) antibodies
were served as negative controls, while anti-human
CD90 (BD Bioscience, USA), CD105 (IMMUNOSTEP,
Spain), CD73 (BD Bioscience, USA) and CD146 (BD
Bioscience, USA) were used as specific antibodies. Cells
were evaluated with a FACS Calibur apparatus (Becton
Dickinson, USA). Finally, the analysis was done using
FlowJo 7.6 software.
Mashayekhi P., Noruzinia M., Zeinali S, & Khodaverdi S. (2019). Endometriotic Mesenchymal Stem Cells Epigenetic Pathogenesis: Deregulation of miR-200b, miR-145, and let7b in A Functional Imbalanced Epigenetic Disease. Cell Journal (Yakhteh), 21(2), 179-185.
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5

Mesenchymal Cell Characterization by Flow Cytometry

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Mesenchymal cells were digested enzymatically by trypsin and centrifuged to obtain the cell pellet, which was resuspended again in 5% FBS and incubated in a dark place for 30 minutes at 4°C in monoclonal antibodies. Negative control antibodies (human CD45, BD Biosciences, and CD34, IMMUNOSTEP, Spain) and specific antibodies (anti-human CD90 (BD Biosciences, USA), CD105 (IMMUNOSTEP, Spain), CD73 (BD Biosciences, USA), and CD146 (BD Biosciences, USA)) were used to cells' assessment by a FACS Calibur apparatus (Becton Dickinson, USA).
Bahramy A., Zafari N., Izadi P., Soleymani F., Kavousi S, & Noruzinia M. (2021). The Role of miRNAs 340-5p, 92a-3p, and 381-3p in Patients with Endometriosis: A Plasma and Mesenchymal Stem-Like Cell Study. BioMed Research International, 2021, 5298006.
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6

Multiparametric Immune Cell Profiling

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Fluorochrome-conjugated antibodies were purchased from BioLegend (CD3 [catalog 317330], CD56 [catalog 362510], NKG2D [catalog 320806], CD96 [catalog 338418], granzyme B [catalog 515408], perforin [catalog 308112], IFN-γ [catalog 502538], TNF-α [catalog 502932], CD107a [catalog 328606], TIM-3 [catalog 345018], LAG-3 [catalog 369320], Ki67 [catalog 350515], human CD45 [catalog 304041], mouse CD45 [catalog 103149]); BD Biosciences (anti-CD3 [catalog 562406], CD16 [catalog 560717]); R&D Systems (TIGIT [catalog FAB7898A], TRAIL [catalog FAB687G]); Invitrogen (Fas-L [catalog 12-9919-42]; and Miltenyi Biotec (PD-1 [catalog 130-117-384], isotype IgG2b-PE [catalog 130-092-215], NKG2A [catalog 130-113-563]).
Myers J.A., Schirm D., Bendzick L., Hopps R., Selleck C., Hinderlie P., Felices M, & Miller J.S. (2022). Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion. JCI Insight, 7(15), e150079.
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