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Fiji imagej software

Manufactured by Olympus
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Fiji-ImageJ is an open-source software platform for scientific image analysis. Its core function is to provide a comprehensive set of tools and algorithms for processing, analyzing, and visualizing various types of digital images, including microscopy data. The software is designed to be extensible and customizable, allowing users to develop and integrate additional plugins and macros to suit their specific needs.

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Lab products found in correlation

Standard matrigel, by BD (1 mentions) Digital camera, by Olympus (1 mentions) Filmtracer live dead biofilm viability kit, by Thermo Fisher Scientific (1 mentions)

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ImageJ (93 citations) ImageJ software (79 citations) Fiji software (6 citations)

2 protocols using fiji imagej software

1

In vitro Angiogenesis and Wound Healing

Cited 1 time
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In vitro tubulogenesis and wound healing assays were performed as previously described [47 (link)]. Briefly, for tube formation assays, HUVECs were seeded on 24-well plates previously covered with 120 μL standard Matrigel (BD Bioscience, San Jose, CA, USA) diluted 1:1 in serum-free RPMI medium or MΦ supernatants for 3 h and 6 h, as indicated. Images were taken with an Olympus digital camera, and quantification of closed tubes (tubular structures/microscopic field) was performed using Fiji-ImageJ software. In vitro scratch wounds were created by scraping confluent HUVEC monolayers in 24-well plates with a sterile pipette tip. Fresh EBM2 medium supplemented with 2% FBS and EGM2 SingleQuots diluted 1:1 with MΦ supernatants was added, and samples were incubated for up to 10 h. Endothelial cell migration into the denuded area was monitored at different times and recorded at 8 h post-wound. The ImageJ program [70 (link)] was used to quantify the wound healing process.
Aristorena M., Gallardo-Vara E., Vicen M., de Las Casas-Engel M., Ojeda-Fernandez L., Nieto C., Blanco F.J., Valbuena-Diez A.C., Botella L.M., Nachtigal P., Corbi A.L., Colmenares M, & Bernabeu C. (2019). MMP-12, Secreted by Pro-Inflammatory Macrophages, Targets Endoglin in Human Macrophages and Endothelial Cells. International Journal of Molecular Sciences, 20(12), 3107.
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2

Biofilm Inhibition Evaluation of Hydrogels

Cited 1 time
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UAMS-1 biofilms were formed on glass chamber coverslips. Samples were prepared using established biofilms treated with the hydrogels (as compared to use of the hydrogels applied prophylactically) to evaluate the potential for inhibition of biofilm formation. Following exposure to each treatment for 24 h, biofilms were then rinsed with sterile water and prepared for observation by CLSM.41 (link), 43 –45 (link) After the sterile water wash, samples from each treatment group were multi-stained with FilmTracer LIVE/DEAD Biofilm Viability Kit and either Wheat Germ Agglutinin (WGA) Alexa Fluor Conjugate 488 or 555 (Invitrogen), according manufacturer specifications. The sample was rinsed with sterile water after stain removal and kept hydrated for observation using an Olympus FluoView 1200 Laser Scanning Confocal Microscope (Olympus America Inc., Melville, NY). The CLSM images were acquired under 10X magnifications, and image analysis/processing was performed using Olympus FluoView and FIJI/Image-J software.
Qian Y., Altamimi A., Yates S.A., Sarkar S., Cochran M., Zhou M., Levi-Polyachenko N, & Matson J.B. (2020). H2S-Releasing Amphiphilic Dipeptide Hydrogels Are Potent S. aureus Biofilm Disruptors. Biomaterials science, 8(9), 2564-2576.
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