Total protein was extracted from cells or the xenograft tumors, and examined by Western blot following our previous published protocols20 (link). Briefly, cells were lysed in the RIPA buffer and centrifuged at 4 °C. Equal amounts of protein (20–35 μg) were separated with SDS–PAGE and transferred onto PVDF membranes. Membranes were blocked and then incubated overnight with primary antibodies: MARK1 (#3319, Cell Signaling, Danvers, MA, USA), MARK2 (#9118, Cell Signaling), MARK4 (#4834, Cell Signaling), MARK3 (#9311, Cell Signaling), Tau (ab64193, Abcom, Cambridge, UK), p-Tau (ab92627, Abcom), and Cleaved-caspase 3 (#9661, Cell Signaling). After washes, the membranes were incubated with secondary antibodies. The immunoreactive bands were visualized using BeyoECL Plus purchased from Beyotime Institute of Biotechnology (Nantong, China) by enhanced chemiluminescence system (Pittsburgh, PA, USA).
Mark2
Manufactured by Cell Signaling Technology
Sourced in United States
MARK2 is a highly specific and potent inhibitor of the microtubule-associated protein/microtubule affinity-regulating kinase 2 (MARK2) enzyme. MARK2 is a serine/threonine protein kinase that plays a role in the regulation of microtubule dynamics and cell polarity.
Automatically generated - may contain errors
Lab products found in correlation
Mark3, by Cell Signaling Technology (2 mentions)
Mark4, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Beyoecl plus, by Beyotime (1 mentions)
Paxillin, by BD (1 mentions)
Total erk1 2, by Cell Signaling Technology (1 mentions)
Total acc, by Cell Signaling Technology (1 mentions)
P p90rsks380, by Cell Signaling Technology (1 mentions)
Pfak y925, by Cell Signaling Technology (1 mentions)
P ulk1s555, by Cell Signaling Technology (1 mentions)
P paxillin y118, by Cell Signaling Technology (1 mentions)
Paccs79, by Cell Signaling Technology (1 mentions)
Nuak1, by Cell Signaling Technology (1 mentions)
P src family y416, by Cell Signaling Technology (1 mentions)
Ampk alpha, by Cell Signaling Technology (1 mentions)
Phospho fak y397, by Abcam (1 mentions)
Wnt5a b, by Cell Signaling Technology (1 mentions)
P mek1 2, by Cell Signaling Technology (1 mentions)
P s6k, by Cell Signaling Technology (1 mentions)
Myc tag, by Cell Signaling Technology (1 mentions)
2 protocols using mark2
1
Western Blot Analysis of Protein Markers
2
Signaling Pathway Antibody Analysis
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