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3 protocols using nb110 57130

1

Immunofluorescence Analysis of Meiotic Proteins

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The following antibodies were used: rabbit antibodies against TERB1 (Shibuya et al., 2014 (link)), TERB2 (Shibuya et al., 2015 (link)), MAJIN (Shibuya et al., 2015), SYCE3 (Zhang et al., 2019 (link)), TRF2 (Novus Biologicals; NB110–57130), γH2AX (Abcam; ab11174), and SMC3 (Abcam; ab9263); mouse antibodies against TRF1 (Shibuya et al., 2014 (link)), β-actin (Sigma; A2228–100UL), and MLH1 (BD Biosciences; 51–1327GR); rat antibody against RPA2 (Cell Signaling Technology; 2208); guinea pig antiserum against histone H1T (Inselman et al., 2003 (link)); goat antibody against Lamin B (Santa Cruz Biotechnology; sc-6216); and chicken antibody against SYCP3 (Zhang et al., 2019 (link)).
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2

Chromatin Immunoprecipitation and ELISA Assays

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For chromatin immunoprecipitation, anti-TRF2 antibody (Novus Biologicals, NB110-57130), anti-H3 (ab1791, Abcam), anti-H3K4me1 (ab8895, Abcam), anti-H3K4me3 (ab8580, Abcam), anti-H3K27me3 (ab192985, Abcam), and anti-rabbit IgG/anti-mouse IgG (Millipore) were used. For ELISA, anti-TRF2 antibody (Novus Biologicals NB110-57130) was used. For Western blotting, the following primary antibodies were used: TRF2 (Novus, NB110-57130), DDK (Sigma, F1804), and GAPDH (Santa Cruz Biotechnology, G-9).
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3

Immunofluorescence Analysis of Meiotic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: rabbit antibodies against TERB1 (Shibuya et al., 2014 (link)), TERB2 (Shibuya et al., 2015 (link)), MAJIN (Shibuya et al., 2015), SYCE3 (Zhang et al., 2019 (link)), TRF2 (Novus Biologicals; NB110–57130), γH2AX (Abcam; ab11174), and SMC3 (Abcam; ab9263); mouse antibodies against TRF1 (Shibuya et al., 2014 (link)), β-actin (Sigma; A2228–100UL), and MLH1 (BD Biosciences; 51–1327GR); rat antibody against RPA2 (Cell Signaling Technology; 2208); guinea pig antiserum against histone H1T (Inselman et al., 2003 (link)); goat antibody against Lamin B (Santa Cruz Biotechnology; sc-6216); and chicken antibody against SYCP3 (Zhang et al., 2019 (link)).
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