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1100 series lc ms

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The Agilent 1100 Series LC-MS is a liquid chromatography-mass spectrometry system. It is designed to provide reliable and accurate analysis of a wide range of compounds. The system combines a high-performance liquid chromatography (HPLC) module with a mass spectrometer, allowing for the separation, identification, and quantification of complex samples.

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Lab products found in correlation

Vnmrs 500, by Agilent Technologies (1 mentions) Mercury 300 nmr, by Agilent Technologies (1 mentions) Chemstation, by Agilent Technologies (1 mentions) Human serum, by Merck Group (1 mentions) C18 spin column, by Thermo Fisher Scientific (1 mentions) Sb c18 column, by Agilent Technologies (1 mentions) Av 400 spectrometer, by Bruker (1 mentions) Av 400 instrument, by Bruker (1 mentions) Mercury, by Agilent Technologies (1 mentions) Lc msd sl, by Agilent Technologies (1 mentions) Avance drx 500, by Agilent Technologies (1 mentions)

5 protocols using 1100 series lc ms

1

Analytical Techniques for Chemical Characterization

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Chemicals were used without any further purification and purchased from common suppliers. Geduran silica gel 60A was generally used as stationary phase for column chromatography, where necessary. A HPMS 6890-5973 MSD spectrometer was used for mass spectrometry analyses equipped with a HP ChemStation or with an Agilent LC–MS 1100 Series. For exact mass analyses, in particular, we employed an LC–MSD Trap System VL spectrometer equipped with electrospray ionization (ESI). Nuclear Magnetic Resonance (NMR) spectra were recorded in the specific deuterated solvent (as indicated in each procedure) using Agilent VNMRS500 or Varian Mercury 300 NMR instruments. Chemical shifts (δ) are reported as parts per million (ppm) and coupling constants (J) in Hertz (Hz). A selection of spectra was reported in the Supplementary Material File (Figure S1). Melting points of solid compounds (uncorrected) were determined in open capillaries on a Gallenkamp electrothermal apparatus. The purity of all tested compounds, based on the panel of analyses performed was estimated as >95%.
Carocci A., Barbarossa A., Leuci R., Carrieri A., Brunetti L., Laghezza A., Catto M., Limongelli F., Chaves S., Tortorella P., Altomare C.D., Santos M.A., Loiodice F, & Piemontese L. (2022). Novel Phenothiazine/Donepezil-like Hybrids Endowed with Antioxidant Activity for a Multi-Target Approach to the Therapy of Alzheimer’s Disease. Antioxidants, 11(9), 1631.
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2

Quantification of COPA Compounds in Human Serum

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Standard solutions of COPA compounds (1.0 mM) were prepared in DMSO. KMS5 (4 μL) was added to a mixture of H2O (96 μL) and human serum (100 μL; Sigma Aldrich), and incubated at 37°C. A 20 μL of plasma was aliquoted from the incubation solution at 0, 24, 48, 72, 96, 120 and 144 h time points. A 1 µL of 0.2 mM internal standard COPA compound was added to the mixture and immediately applied to C18-spin column (Thermo Scientific). The COPA compounds were purified following supplier's protocol and eluted with a 40 μL of an elution solution (80% acetonitrile, 20% H2O). Resulting solution was diluted with 0.1% formic acid and analyzed by LC-MS ((LC/MS 1100 Series equipped with SBC18 column from Agilent)) with a linear gradient of (5% acetonitrile, 0.05% formic acid/aqueous solution) to (80% acetonitrile, 0.05% formic acid/aqueous solution).
Sarkar M., Liu Y., Morimoto J., Peng H., Aquino C., Rader C., Chiorazzi N, & Kodadek T. (2014). Recognition of Antigen-Specific B Cell Receptors From Chronic Lymphocytic Leukemia Patients By Synthetic Antigen Surrogates. Chemistry & biology, 21(12), 1670-1679.
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3

Spectroscopic Characterization of Compounds

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All the reagents were commercially available and used without further purification. Melting points were determined on an X-4 binocular microscope melting point apparatus. 1H NMR and 13C NMR spectra were recorded on a Bruker AV-400 spectrometer, using tetramethylsilane (TMS) as the internal standard and chemical shifts (δ) were expressed in ppm. Mass spectra were obtained by an Agilent 1100 series LC-MS. Elemental analyses were performed on a Vario EL III (Germany) instrument.
Lin D., Wang L., Yan Z., Ye J., Hu A., Liao H., Liu J, & Peng J. (2018). Semi-synthesis, structural modification and biological evaluation of 5-arylbenzofuran neolignans. RSC Advances, 8(60), 34331-34342.
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4

Analytical Characterization of Organic Compounds

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All solvents were analytical reagents (ARs), commercially available, and used without further purification. The reaction systems were monitored by thin-layer chromatography with silica gel precoated glass and fluorescent indicator, and the removal of solvent was carried out with a rotary evaporator and vacuum pump. As previously reported, proton (1H) and carbon (13C) NMR spectra were recorded on a Bruker AV-400 instrument and are reported in ppm relative to tetramethylsilane (TMS) and referenced to the solvent in which the spectra were collected. Low-resolution and high-resolution mass spectral (MS) data were determined on an Agilent 1100 Series LC-MS with UV detection at 254 nm and a low-resonance electrospray mode (ESI). All target compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). The HPLC analysis was performed on a Waters 2695 HPLC system equipped with a Kromasil C18 column (4.6 mm × 250 mm, 5 μm).
Yuan C., Yan J., Song C., Yang F., Li C., Wang C., Su H., Chen W., Wang L., Wang Z., Qian S, & Yang L. (2019). Discovery of [1,2,4]Triazole Derivatives as New Metallo-β-Lactamase Inhibitors. Molecules, 25(1), 56.
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5

Synthesis and Characterization of 1-Acylamino-2,2-Dichloroethenyltriphenylphosphonium Chlorides

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1H, 13C, 31P NMR spectra were obtained on a Bruker AVANCE DRX‐500 or Varian Mercury (400, 125, 151, 162 MHz, respectively) spectrometer (TMS as internal reference or 85 % phosphoric acid as external reference) in DMSO‐d6. IR spectra were recorded on a Vertex 70 spectrometer in KBr pellets. Mass spectra were recorded on an Agilent 1100 Series LC‐MS system equipped with a diode array detector Agilent LC\MSD SL (atmospheric pressure chemical ionization). Elemental analysis was carried out in the Analytical Laboratory of the Institute of Bioorganic and Petrochemistry of the National Academy of Sciences of Ukraine by manual methods. The carbon and hydrogen contents were determined using the Pregl gravimetric method, while nitrogen was determined using the Duma's gasometrical micromethod. Chlorine content was determined by the mercurometric method, phosphorus content was determined by the colorimetric method and sulfur content by the Scheininger titrimetric method. M. p. was determined on a Fisher–Johns apparatus and are uncorrected. All reagents and solvents were purchased from commercial sources were used.
1‐Acylamino‐2,2‐dichloroethenyltriphenylphosphonium chlorides I were synthesized according to the method described in the article.[13]
Brusnakov M., Golovchenko O., Velihina Y., Liavynets O., Zhirnov V, & Brovarets V. (2022). Evaluation of Anticancer Activity of 1,3‐Oxazol‐4‐ylphosphonium Salts in Vitro. Chemmedchem, 17(20), e202200319.
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