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Anti ikaros

Manufactured by Active Motif
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Anti-Ikaros is a laboratory reagent that specifically binds to the Ikaros protein, a transcription factor involved in lymphocyte development and function. This product can be used in various research applications to detect, quantify, or study the Ikaros protein and its role in cellular processes.

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Lab products found in correlation

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2 protocols using anti ikaros

1

Histone Modifications and Chromatin Interactions

Cited 2 times
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Rabbit polyclonal to H1, HMGN1, HMGN2, and H3 were from our laboratory, anti H3K27ac (Abcam#ab4729), anti H3K27me3 (Abcam#ab6002), monoclonal anti H1(Milipore-Sigma #05-457), anti CEBPB (Abcam#ab32358), Anti-Brd3 (Active Motif #61489), Anti-Brd4 (Bethyl Laboratories #A301-985A100), Anti-CEBPB (Abcam #ab32358), Anti-CTCF (EMD Millipore #07-729), Anti-Ets1 (Active Motif #39580), Anti-Ikaros (Active Motif #39355), Anti-Irf8 (Bethyl Laboratories #A304-027A), Anti-Klf4 (Abcam #106629), Anti-Nanog (Active Motif #61419), Anti-Oct4 (Abcam #ab19857), Anti-p300 (Active Motif #61401), Anti-Pax5 (Abcam #183575), Anti-Sox2 (Abcam #97959).
The following recombinant mononucleosomes were purchased from Active Motif: unmodified (#81070); H3K27me3 modified (#81834), H3K27ac modified (#81077).
Wild type and HMGN DKO mouse embryonic fibroblasts, embryonic stem cell lines55 (link) and resting B cells56 (link) were as previously described. Peptides Histone H3 (23–34) peptide, KAARKSAPATGG and Histone H3K27ac (23–34) peptide, KAAR - K(Ac)—SAPATGG were from AnaSpec, Inc.
Zhang S., Postnikov Y., Lobanov A., Furusawa T., Deng T, & Bustin M. (2022). H3K27ac nucleosomes facilitate HMGN localization at regulatory sites to modulate chromatin binding of transcription factors. Communications Biology, 5, 159.
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2

Immunoblot and Intracellular Antibody Analysis

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The following antibodies were used for immunoblot analysis: anti-Ikaros (mouse mAb clone 4E9; from K. Georgopoulos, Harvard University), anti-TBP (mouse mAb clone 3TF1-3G3; Active Motif) and for cell stimulation or intracellular staining: anti-Igβ (Armenian hamster IgG2 mAb clone HM79; from Hajime Karasuyama, Tokyo), anti-TCRγδ (Armenian hamster IgG2 mAb clone GL-3), anti-pAkt (S473, D9E; Cell Signaling Technology), anti-pErk1/2 (T202/Y204, 20A; BD Bioscience), anti-Aiolos (8B2; eBioscience), anti-Ikaros (2A9, Biolegend; Supplementary Fig. 1f,g) and the rabbit polyclonal anti-Ikaros antibody (5443; Cell Signaling; Supplementary Fig. 8d,e).
Schwickert T.A., Tagoh H., Gültekin S., Dakic A., Axelsson E., Minnich M., Ebert A., Werner B., Roth M., Cimmino L., Dickins R.A., Zuber J., Jaritz M, & Busslinger M. (2014). Stage-specific control of early B cell development by the transcription factor Ikaros. Nature immunology, 15(3), 283-293.
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