Igm apc cy7

Manufactured by BioLegend

Sourced in United States

IgM APC-Cy7 is a fluorescently-labeled antibody that binds to the IgM isotype of immunoglobulins. It is designed for use in flow cytometry applications to identify and quantify IgM-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

APC-Cy7 (106 citations) IgM-APC (10 citations) Anti‐IgM APC‐Cy7 (3 citations) Anti-human IgM-APC/Cy7 (2 citations)

4 protocols using igm apc cy7

Multiparameter Flow Cytometry of Hematopoietic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions obtained from colony forming assays or hematopoietic organs from mice were surface stained with monoclonal antibodies. Antibodies used for analysis of human immature stem and progenitor cells from colony forming assays: CD34 PE-Cy7(581), CD38 APC (HIT2), CD10 PE/PeCy5/APC (HI10a), CD45RA PerCP-Cy5.5 (HI100), CD90 APC-Cy7 (5E10) (Biolegend). Antibodies for erythroid cells from colony assays and HEMA: CD117 PE-Cy7 (104D2), CD71 APC/APC-Cy7 (CY1G4) (Biolegend), CD235a BV421 (HIR2) (BD biosciences). Antibodies for myeloid cells from colony forming assays: CD34 PE-Cy7 (581), CD33 PE/PerCP-Cy5.5 (WM53), CD14 APC (M5E2), CD115 BV421 (g-4D2-1E4), CD15 PeCy5 (W6D3), CD66b PerCP-Cy5.5 (G10F5) (Biolegend). Antibodies for megakaryocyte populations: CD41 PerCP-Cy5.5 (HIP8), CD61 APC (VI-PL2) (Biolegend). Antibodies for overall human and human myeloid cells in xenograft assay: CD45 Biotin (HI30), CD34 PE-Cy7/APC-Cy7 (581), CD33 PE/PerCP-Cy5.5 (WM53), CD15 PeCy5 (W6D3). Antibodies for human B cells in xenograft assay: CD19 PE-Cy7 (HIB19), IgM APC-Cy7 (MHM-88), CD10 APC (HI10a) (Biolegend). Antibody to detect murine cells in xenograft assay: CD45 PE-Cy7/PerCP-Cy5.5 (30-F11) (Biolegend). Streptavidin APC/V450 (Biolegend) were used as secondary antibodies. BD LSRFortessa was used for flow cytometry. Analyses were performed using FlowJo.

Afreen S., Bohler S., Müller A., Demmerath E.M., Weiss J.M., Jutzi J.S., Schachtrup K., Kunze M, & Erlacher M. (2020). BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells. Cell Death & Disease, 11(1), 8.

+ Open protocol

+ Expand

Immunophenotyping of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells analysed were isolated from MLN, spleen or ileum. For staining cell numbers were adjusted to 1 × 10⁶ cells/well. Cells were stained with IgM-APC-Cy7 (1:1000; Biolegend, San Diego, CA, USA), CD19-AF700 (1:1000; Biolegend), B220-VioBlue (1:500, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), granzyme B-FITC (1:100; eBioscience, San Diego, CA, USA), CD8-PE-Cy7 (1:3000; Biolegend) or CD3-APC-Cy7 (1:1000; Biolegend) antibody. For all dilutions MACS buffer was used. Cells were analysed in the Gallious Flow Cytometer (Beckman Coulter).

Basic M., Buettner M., Keubler L.M., Smoczek A., Bruesch I., Buchheister S, & Bleich A. (2018). Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation. Scientific Reports, 8, 719.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry of Murine Hematopoietic Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions obtained from colony-forming assays or hematopoietic organs from mice were surface stained with monoclonal antibodies: CD34 PE-Cy7(581), CD38 APC (HIT2), CD10 PE/APC (HI10a), CD45RA PerCP-Cy5.5 (HI100), CD90 APC-Cy7 (5E10), CD117 PE-Cy7 (104D2), CD71 APC (CY1G4), CD33 PE (WM53), CD14 APC (M5E2), CD115 BV421 (4D2-1E4), CD15 PeCy5 (W6D3), CD66b PerCP-Cy5.5 (G10F5), CD19 PE-Cy7 (HIB19), IgM APC-Cy7 (MHM-88), CD45 Biotin (HI30), CD45 PE-Cy7/V500 (30-F11) (Biolegend), and CD235a BV421 (HIR2) (BD Biosciences). Streptavidin PerCP-Cy5.5/V450 (Biolegend) was used as a secondary antibody. BD LSRFortessa and FlowJo were used for flow cytometry and analyses, respectively. The gating strategy was published earlier.^{31 (link)}

Bohler S., Afreen S., Fernandez-Orth J., Demmerath E.M., Molnar C., Wu Y., Weiss J.M., Mittapalli V.R., Konstantinidis L., Schmal H., Kunze M, & Erlacher M. (2020). Inhibition of the anti-apoptotic protein MCL-1 severely suppresses human hematopoiesis. Haematologica, 106(12), 3136-3148.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry for Murine B Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

For flow cytometry, 1 × 10⁶ single cells from PL, BM, and spleen were incubated with anti‐mouse CD16/32 (BioLegend) for 15 min to block unspecific Fc‐binding sites. Subsequently, cells were fluorescently stained in two mixes to identify B‐1 cells, CD138⁺ cells, splenic B‐2 cells, and BM subpopulations: B220‐FITC, anti‐CD5‐PerCP‐Cy5.5, anti‐IgD‐V450, anti ‐IgM‐APC‐Cy7, anti‐CD43‐PE, anti‐CD138‐APC and anti‐CD23‐BV510, anti‐B220‐PerCP, anti‐CD21‐FITC, anti‐CD23‐BV510, and GLY7‐PE (BioLegend and BD Bioscience). For identification of PBs and plasma cells (PCs), fluorescent staining was performed using anti‐B220‐FITC, anti‐CD19‐V450, anti‐TACI‐APC, anti‐CD138‐PE, and 7‐AAD for exclusion of dead cells.
To analyze surface immune globulins after in vitro stimulation, splenic B cells were incubated with anti‐CD19‐V450, anti‐CD43‐PE‐Cy7, anti‐CD5‐APC, anti ‐IgM‐APC‐Cy7, anti‐IgD‐PerCP and anti‐IgG₁‐PE (LPS/IL‐4) or with anti‐CD19‐APC‐Cy7, anti‐CD43‐PE, anti‐CD5‐APC, anti‐IgA‐BV421, anti‐IgG_2ab‐BB700, and anti‐IgG₃‐PE‐Cy7 (LPS only) (Biolegend and BD Bioscience). Proliferation was analyzed via carboxyfluorescein succinimidyl ester‐staining.
All measurements were performed at the BD FACS Canto II and biaxial gating was done with FlowJo Version 10. For flow cytometry gating strategies the reader is referred to Figure S2.

Jaufmann J., Tümen L, & Beer‐Hammer S. (2021). SLy2‐overexpression impairs B‐cell development in the bone marrow and the IgG response towards pneumococcal conjugate‐vaccine. Immunity, Inflammation and Disease, 9(2), 533-546.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!