Immunodetection of neuronal markers was carried out as previously described [53 (link), 54 (link)]. Primary antibodies used were: rabbit anti-doublecortin (Cell Signaling Technology Inc.), monoclonal anti-NeuN (Millipore), goat anti-FZD1 (R&D Systems), goat anti-FZD1 (LifeSpan Biosciences, Inc.), rabbit anti-SOX2 (Cell Signaling Technology Inc.), monoclonal anti-GFAP (Sigma-Aldrich), monoclonal anti-Nestin (Millipore), rabbit anti-Ki67 (Abcam). As secondary antibodies, Alexa (Molecular Probes) and DyLight (Abcam) conjugated antibodies were used. NucBlue (Life Technologies) was used as nuclear dye. Slices were mounted on gelatin-coated slides with Fluoromont-G (Electron Microscopy Sciences). Double-labeled sections were analyzed by confocal laser microscopy (Olympus FV 1000). Image analysis and z-projections were made with ImageJ software (NIH, USA).
Rabbit anti doublecortin
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-doublecortin is a primary antibody produced in rabbits that specifically binds to the doublecortin protein. Doublecortin is a microtubule-associated protein that plays a crucial role in neuronal migration and differentiation during brain development.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti ki67, by Abcam (4 mentions)
Monoclonal anti neun, by Merck Group (3 mentions)
Mouse anti neun, by Merck Group (3 mentions)
Rat anti brdu, by Abcam (3 mentions)
Monoclonal anti nestin, by Merck Group (2 mentions)
Rat anti brdu, by Bio-Rad (2 mentions)
Lsm 700, by Zeiss (2 mentions)
Rabbit anti sox2, by Cell Signaling Technology (1 mentions)
Monoclonal anti gfap, by Merck Group (1 mentions)
Fluoromont g, by Electron Microscopy Sciences (1 mentions)
Goat anti fzd1, by R&D Systems (1 mentions)
Nucblue, by Thermo Fisher Scientific (1 mentions)
Fv1000, by Olympus (1 mentions)
Goat anti sox2, by Santa Cruz Biotechnology (1 mentions)
Chicken anti egfp, by Abcam (1 mentions)
Rabbit anti olig2, by Merck Group (1 mentions)
Rat anti gfap, by Thermo Fisher Scientific (1 mentions)
Mouse anti mash1, by BD (1 mentions)
C11440 hamamatsu camera, by Hamamatsu Photonics (1 mentions)
Spinning disk confocal microscope system, by Zeiss (1 mentions)
6 protocols using rabbit anti doublecortin
1
Immunodetection of Neuronal Markers
2
Immunocytochemistry of Frozen Tissue Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunocytochemistry of frozen tissue sections was performed essentially as described (Voronova et al., 2017 (link)) and as detailed in the Supplemental Experimental Procedures . The following primary antibodies were used in this study: goat anti-SOX2 (Santa Cruz, 1:250, catalog no. sc-17320), chicken anti-eGFP (Abcam, 1:1,000, catalog no. ab13970), rat anti-GFAP (Invitrogen, 1:300, catalog no. 130300), mouse anti-MASH1 (BD Pharmingen, 1:1,000, catalog no. 556604), rabbit anti-KI67 (Abcam, 1:250, catalog no. ab15580), rabbit anti-DOUBLECORTIN (Cell Signaling Technology, 1:500, catalog no. 4604), rabbit anti-OLIG2 (Millipore, 1:1,000, catalog no. AB9610), goat anti-PDGFRA (R&D Systems, 1:250, catalog no. AF1062), mouse anti-NEUN (Millipore, 1:500, catalog no. MAB377) and rat anti-BrdU (AbD Serotec, 1:300, catalog no. OBT0030). Fluorescently labeled highly cross-absorbed secondary antibodies were purchased from Jackson ImmunoResearch and used at a dilution of 1:1,000. z-Stacked images were collected using a Quorom Spinning Disk confocal microscope system or a Zeiss Axio Imager M2 system with an X-Cite 120LED light source and a C11440 Hamamatsu camera. Images were taken with an optical slice thickness of 0.3 μm and projected z-stacked images are shown.
3
Immunodetection of Neurogenesis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunodetection of BrdU and neuronal markers in tissue sections was carried out as previously described in [26 (link)]. Primary antibodies used were rat anti-BrdU (Abcam), rabbit anti-doublecortin (Cell Signaling Technology Inc.), monoclonal anti-NeuN (Millipore, Billerica, MA, USA), rabbit anti-Ki67 (Abcam), goat anti-NeuroD1 (Santa Cruz Biotechnology, Inc.), monoclonal anti-Nestin (Millipore), Alexa (Molecular Probes, Life Technologies), and DyLight (Abcam) conjugated secondary antibodies were used. Slices were mounted on gelatin-coated slides with Fluoromount-G (Electron Microscopy Sciences).
4
Immunostaining of Neural Markers
5
Immunodetection of Neurogenesis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunodetection of BrdU and neuronal markers in tissue sections was carried out as previously described (Abbott et al., 2013 (link)). Primary antibodies used were: rat anti-BrdU (Abcam), rabbit anti-Doublecortin (Cell Signaling Technology Inc., Beverly, MA, USA), monoclonal anti-NeuN (Millipore, Billerica, MA, USA) and rabbit anti-Ki67 (Abcam). As secondary antibodies, Alexa (Molecular Probes) and DyLight (Abcam) conjugated antibodies were used. BrdU and Ki67 positive cells were counted using a fluorescence microscope (Olympus BX51, Tokyo, Japan) as described (Abbott et al., 2013 (link)). Double-labeled sections were analyzed by confocal laser microscopy (Olympus FV 1000). Image analysis and z-projections were made with ImageJ software (NIH, USA).
6
Comprehensive Immunohistochemical Analysis of Neural Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistochemistry was performed as previously described [32] . The following antibodies and final dilutions were used. Primary antibodies: rabbit anti-Cav1.2 (1:500, Sigma Aldrich), rat anti-BrdU (1:500, Serotec), mouse anti-Nestin (1:300, Abcam), goat anti-Doublecortin (1:200, Santa Cruz), rabbit-anti Doublecortin (1:300, Cell Signaling), guinea pig anti-GFAP (1:500, Progen), mouse anti-NeuN (1:500, Millipore), goat anti-PCNA (1:250, Santa Cruz), chicken anti-GFP (1:500, Invitrogen). Secondary antibodies: donkey anti-goat, -rabbit,chicken Alexa 488, donkey anti-guinea pig Alexa 568, donkey anti-goat, -guinea pig, -mouse Alexa 647 (all 1:1000, Invitrogen), donkey anti-mouse, -goat, -rat biotinylated (all 1:500, Jackson Immuno Research). Nuclear counterstaining was performed with 4', 6'-diamidino-2phenylindoledihydrochloride hydrate at 0.25 μg/μl (DAPI; Sigma). Chromogenic immunodetection was photodocumented using a Zeiss Axioplan microscope (Zeiss, Germany) equipped with the ZeissAxioVision imaging system. Epifluorescence observation was performed on a confocal scanning laser microscope (LSM 700, Zeiss) with LSM software (ZEN 2011).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!