Rabbit anti ire1

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-IRE1 is a polyclonal antibody that recognizes the Inositol-Requiring Enzyme 1 (IRE1) protein. IRE1 is a key component of the unfolded protein response (UPR) pathway, which is activated in response to endoplasmic reticulum (ER) stress. This antibody can be used for the detection and analysis of IRE1 in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti-IRE1 (13 citations)

2 protocols using rabbit anti ire1

Comprehensive Antibody Panel for Autophagy and UPR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit anti-LC3B (#3868), rabbit anti-ATG5 (#12994), rabbit anti-ATG7 (#8558), rabbit anti-Beclin-1 (#3495), rabbit anti-ATG16L1 (#8089), rabbit anti-BIP (#3177), rabbit anti-ATF6 (#65880), rabbit anti-ATF4(#11815), rabbit anti-XBP1 (#12782), rabbit anti-PERK (#5683), rabbit anti-IRE1 (#3294), rabbit anti-p-eif2α (#3398), rabbit anti-FIP200 (#12436), and mouse anti-CHOP (#2895) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) (dilution concentration 1:1000). Rabbit anti-p-IRE1 (PA1-16927) was purchased from Invitrogen (Waltham, MA, USA) (dilution concentration 1:1000). Mouse anti-p62 (18420-1-AP), mouse anti-HA-tag (66006-2-Ig), and mouse anti-β-actin (66009-1-Ig) were purchased from Proteintech (Wuhan, China) (dilution concentration 1:5000). HRP-conjugated goat anti-mouse (G1214) and goat anti-rabbit (G1213) were purchased from Servicebio (Wuhan City, China) (dilution concentration 2:5000). Alexa Fluor 568 goat anti-mouse IgG, IgM (H + L) (A-11004) and Alexa Fluor 647 goat anti-mouse-IgG (H + L) (A-21445) were purchased from Thermo Fisher (Waltham, MA, USA) (dilution concentration 1:500). Immunofluorescence antibodies were diluted in PBS. Immunoblot antibodies were diluted in TBST.

Su W.Q., Yu X.J, & Zhou C.M. (2021). SARS-CoV-2 ORF3a Induces Incomplete Autophagy via the Unfolded Protein Response. Viruses, 13(12), 2467.

+ Open protocol

+ Expand

Molecular Mechanisms of Endoplasmic Reticulum Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used were as follows: mouse anti-XBP1s (Biolegend, 647502), rabbit anti-IRE1 (Cell Signaling, 3294), rabbit anti-PERK (Cell Signaling Technology, 3192), rabbit anti-eIF2α (Cell Signaling Technology, 5324), rabbit anti-p(S51)-eIF2α (Cell Signaling Technology, 3398), mouse anti-ATF6 (CosmoBio, BAM-73-500-EX), rabbit anti-NLRP3 D2P5E (Cell signaling technology, 13158), rabbit anti-NF-κB p65 (D14E12) (Cell signaling technology, 8242), mouse anti-IL-1β (R&D Systems, MAB601), rabbit anti-ASC (Santa Cruz, sc-22514-R), rabbit anti-caspase 1 (Santa Cruz, sc-622), rabbit anti-caspase-1 p10 (Santa Cruz, sc-515), TXNIP (Santa Cruz, sc-166234), and rabbit anti-Actin (Sigma, A2066). Secondary antibodies were horseradish peroxidase-tagged goat anti-mouse (Jackson Laboratories, 115-035-003) and goat anti-rabbit antibodies (Jackson Laboratories, 111-035-003). Tunicamycin (T7765), Phorbol 12-myristate 13-acetate (PMA) (P8139), LPS (L2630), and ATP (A6419) were purchased from Sigma-Aldrich while nigericin (tlrl-nig) was obtained from Invivogen. IRE1 inhibitor MKC8866 was provided by Fosun Orinove.

Talty A., Deegan S., Ljujic M., Mnich K., Naicker S.D., Quandt D., Zeng Q., Patterson J.B., Gorman A.M., Griffin M.D., Samali A, & Logue S.E. (2019). Inhibition of IRE1α RNase activity reduces NLRP3 inflammasome assembly and processing of pro-IL1β. Cell Death & Disease, 10(9), 622.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!