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Anti jnk3

Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States

Anti-JNK3 is a specific antibody that binds to the c-Jun N-terminal kinase 3 (JNK3) protein. JNK3 is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in cellular processes such as apoptosis, differentiation, and inflammation. The Anti-JNK3 antibody can be used in various laboratory techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the JNK3 protein.

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2 protocols using anti jnk3

1

Immunofluorescence Analysis of Hippocampal Synaptic Proteins

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Immunofluorescence experiments were performed according to [73 (link)]. Briefly, hippocampal primary cultures at DIV14 (see above) were fixed in 4% paraformaldehyde (PFA) and 4% sucrose solution for 30 min, followed by permeabilization with phosphate-buffered saline (PBS) at pH 7.4, containing 0.5% Triton X-100, for 3 min. Co-cultures were first blocked for 1 h in PBS containing 1% BSA, 0.2% Triton X-100, and subsequently incubated overnight at 4 °C with primary antibodies in PBS containing 1% BSA and 0.2% Triton X-100. The following antibodies were used: anti-GFP (AbCam Cambridge, UK Ab290), anti-PSD95 (AbCam Cambridge, UK Ab12093), anti-JIP1 (AbCam Cambridge, UK Ab24449), anti-β-arrestin2 (AbCam Cambridge, UK Ab31294), anti-JNK3 (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA #PA5-14421). Cells were finally incubated with secondary antibodies (AlexFluor Antibody, Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at room temperature. A concentration of 2 mg/mL Hoechst (Thermo Fisher Scientific, Waltham, MA, USA, 33342) was used to stain nuclei. ProLong Glass Antifade Mountant (Thermo Fisher Scientific, Waltham, MA, USA) was used as a mounting agent.
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2

Immunoprecipitation and Western Blot Analysis

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Immunoprecipitated proteins were separated by 10% SDS polyacrylamide gel electrophoresis. PVDF membranes were blocked in Tris-buffered saline (5% no-fat milk powder, 0.1% Tween 20), (1 h, RT). Primary antibodies (anti-JNK3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, #PA5-14421, mAB #2305; anti-PSD95 Clone 6G6 Cayman Chemical, Michigan, USA; anti-JIP1 AbCam Cambridge, UK Ab24449; and anti-β-arrestin2, Thermo Fisher Scientific, Waltham, MA, USA, # PA1-732) were diluted in BSA 5%, 0.1% Tween 20 and incubated overnight at 4 °C. Blots were developed using horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA) and the ECL chemiluminescence system (Bio-Rad, Hercules, CA, USA).
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