CA4P (MW: 440.30), Lactobionic acid (LA, MW: 358.3) and cholesterol were purchased from Beijing Innochem Co., Ltd. (China). L-α-phosphatidylcholine, Thiazolyl blue tetrazolium bromide (MTT, MW:414.3), fetal bovine serum (FBS) and 4ʹ,6-Diamidino-2-phenylindole (DAPI, MW:350.2) were obtained from Beijing Solarbio Co., Ltd. (China). DOX was obtained from Shanxi Powerdone Pharmaceutical Co., Ltd (China). DSPE-PEG-NHS was obtained from Xi’an Ruixi Technology Co., Ltd. (China). All other chemicals were of analytical grade.
L α phosphatidylcholine
Manufactured by Solarbio
Sourced in China
L-α-phosphatidylcholine is a naturally occurring phospholipid. It is a major component of cell membranes and plays a role in various biological processes.
Automatically generated - may contain errors
2 protocols using l α phosphatidylcholine
1
Nanocarrier-Mediated Anticancer Drug Delivery
2
Erastin-Loaded Lipid Nanoparticle Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Precisely weighed 120 mg of l -α-phosphatidylcholine (Solarbio, Beijing, China), 40 mg of cholesterol (Solarbio), 20 mg of DSPE-PEG-FA (Shanghai Ponsure Biotechnology, China, Shanghai), and 3 mg of erastin (Sigma-Aldrich, MO, USA) were fully dissolved in 5 mL chloroform and mixed with 3 mL of 1 mg/mL erastin chloroform solution. Chloroform was then removed using a rotary evaporator to form a dry-lipid film at 35 °C under uniform speed. MT1DP (300 nM) dissolved in phosphate-buffered saline (PBS) (pH 7.4, 5 mL) was added to the lipid film and hydrated for 60 min. After the hydration was completed, the cells were sonicated with an ultrasonic cell disruptor and passed through a polyethersulfone membrane with a membrane pore diameter of 450 and 220 nm to decrease the particle size.
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