Guanfacine

Manufactured by Merck Group

Sourced in Germany

Guanfacine is a laboratory equipment product manufactured by Merck Group. It is a chemical compound used in various research and analytical applications. Guanfacine serves as a potent and selective alpha-2 adrenergic receptor agonist, a function that is central to its use in scientific investigations.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Guanfacine hydrochloride

6 protocols using guanfacine

Guanfacine Modulation of Rodent Behavior

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Guanfacine (GUAN, 0.1 and 0.3 mg/kg, i.p [42 (link)–45 (link)]) was obtained from Sigma (St. Louis, MO), dissolved in 0.9% saline (vehicle, VEH), and administered in a volume of 1 mL/kg. Previous literature has found that a range of 0.1 to 0.3 mg/kg is an effective dose of GUAN to reduce locomotion and measures of impulsivity in rodents that are not confounded by sickness and significant sedation [44 (link), 45 (link)]. Therefore, we used a different cohort of mice for each behavioral task and investigated the effects of two doses of GUAN (0.1 and 0.3 mg/kg) that are based on the literature. These doses of GUAN in mice are comparable to the doses used for children with ADHD ([46 (link)]; FDA ref# 3335794). To ensure observers were blind to treatment conditions, vials were letter-coded prior to administration by a person not involved in the running of each experiment.

Lukkes J.L., Drozd H.P., Fitz S.D., Molosh A.I., Clapp D.W, & Shekhar A. (2020). Guanfacine treatment improves ADHD phenotypes of impulsivity and hyperactivity in a neurofibromatosis type 1 mouse model. Journal of Neurodevelopmental Disorders, 12, 2.

+ Open protocol

+ Expand

Osteogenic Differentiation of Cells With ADHD Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ADHD medication modafinil (11.2 µg/mL), atomoxetine (0.9 µg/mL), and guanfacine (17.7 ng/mL) was ordered from Sigma-Aldrich (Steinheim, Germany) and used in max. therapeutic plasma concentration [15 (link),16 (link),17 (link)]. The medication of modafinil solution (1 mg/mL), atomoxetine hydrochloride solution (1 mg/mL) and guanfacine hydrochloride (500 mg) was purchased from Sigma-Aldrich (Steinheim, Germany) and dissolved in sterile DMSO. The osteogenic differentiation medium consisting of ascorbic acid-2-phosphate (200 µM, Cayman Chemical, Ann Arbor, MI, USA), dexamethasone (0.1 µM) and ß-glycerol phosphate (10 mM, Merck, Germany) was used. 10% DMSO was purchased from Merck, Germany.

Wagener N., Lehmann W., Böker K.O., Röhner E, & Di Fazio P. (2022). Chondral/Desmal Osteogenesis in 3D Spheroids Sensitized by Psychostimulants. Journal of Clinical Medicine, 11(20), 6218.

+ Open protocol

+ Expand

Monitoring Cofilin Phosphorylation with Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols

All peptides were synthesized and purified by GenScript, USA, Inc. Peptides containing a 16 aa sequence of the cofilin Ser3 site (MASGVAVSDGVIKVFN, referred to as S3 peptides) or phosphor-Ser3 site [MAS(p)GVAVSDGVIKVFN, referred to as pS3 peptides] were used. These peptides were fused to a TAT-like polyarginine membrane permeability sequence (GRRRRRRRRRRR) to facilitate its entrance into cells and to a biotin molecule to allow detection. The TAT-like peptide (GRRRRRRRRRRR) was used as a control. Antibodies to cofilin (cat #5175S, dilution 1:1000), phosphorylated cofilin (p-cofilin) (cat# 3311S, dilution 1:500) and Myc-Tag (9B11) (cat# #2276S, dilution 1:1000) were purchased from Cell Signaling Technology. HA.11 antibody (cat#901515) was from Biolegend. Clonidine (cat# C7897), guanfacine (cat#G1043), BRL44408 (cat# B4559) were from Sigma-Aldrich, and JP1302 (cat# 26-661-0) and imiloxan (cat# 09-861-0) were purchased from Thermo Fisher Scientific.

Saggu S., Chen Y., Cottingham C., Rehman H., Wang H., Zhang S., Augelli-Szafran C., Lu S., Lambert N., Jiao K., Lu X.Y, & Wang Q. (2022). Activation of a novel α2AAR-spinophilin-cofilin axis determines the effect of α2 adrenergic drugs on fear memory reconsolidation. Molecular Psychiatry, 28(2), 588-600.

+ Open protocol

+ Expand

Pharmacological modulation of behavior

Check if the same lab product or an alternative is used in the 5 most similar protocols

Guanfacine, mecamylamine and physostigmine were purchased from Sigma Aldrich.
Drugs were dissolved in PBS (0.1 M, pH = 7.4) and stock solutions were frozen
until use. For injections, drugs were diluted such that the final solution was injected at
10 ml/kg of body weight. For acute experiments, drugs were injected 30 min before
behavioral testing. For chronic administration, mice receive one injection once a day for
15 days. No injection was given on the day of behavioral testing.

Mineur Y.S., Bentham M.P., Zhou W.L., Plantenga M.E., McKee S.A, & Picciotto M.R. (2015). Antidepressant-like effects of guanfacine and sex-specific differences in c-fos immunoreactivity and paired-pulse ratio in male and female mice. Psychopharmacology, 232(19), 3539-3549.

+ Open protocol

+ Expand

Pharmacological Modulators of Adrenergic Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Guanfacine, oxymetazoline, brimonidine, clonidine (Sigma-Aldrich, Germany), yohimbine (Tocris, Bristol, UK), heparin (Polfa Warszawa S.A., Warsaw, Poland), and thiopental (Sandoz International, France).

Dudek M., Knutelska J., Bednarski M., Nowiński L., Zygmunt M., Mordyl B., Głuch-Lutwin M., Kazek G., Sapa J, & Pytka K. (2015). A Comparison of the Anorectic Effect and Safety of the Alpha2-Adrenoceptor Ligands Guanfacine and Yohimbine in Rats with Diet-Induced Obesity. PLoS ONE, 10(10), e0141327.

+ Open protocol

+ Expand

Nicotine and Guanfacine Administration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nicotine hydrogen tartrate salt (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in saline and injected subcutaneously (s.c.) at free base concentrations of 0.5, 0.75, or 1.0 mg/kg at 10 ml/kg. Guanfacine (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in saline and injected intraperitoneally (0.15 mg/kg injected at 10 ml/kg).

Lee A.M., Calarco C.A., McKee S.A., Mineur Y.S, & Picciotto M.R. (2019). Variability in nicotine conditioned place preference, stress-induced reinstatement, and effects of guanfacine in male and female mice. Genes, brain, and behavior, 19(3), e12601.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!