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Human cxcl9 mig quantikine elisa kit

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The Human CXCL9/MIG Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human CXCL9/MIG levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Human cxcl10 ip 10 quantikine elisa kit dip 100, by R&D Systems (1 mentions) Human cxcl11 1 tac quantikine elisa kit, by R&D Systems (1 mentions) U plex platform, by Mesoscale (1 mentions) Infinite 200, by Tecan (1 mentions) Human cxcl10 ip 10 quantikine elisa kit, by R&D Systems (1 mentions)

4 protocols using human cxcl9 mig quantikine elisa kit

1

Quantification of CXCL Chemokines in Cancer Cells

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Cancer cell supernatants were obtained after in vitro culture under serum deprivation conditions. CXCL9, CXCL10 and CXCL11 were quantified using the human CXCL9/MIG Quantikine ELISA Kit (DCX900, R&D Systems, Minnesota, USA), human CXCL10/IP‐10 Quantikine ELISA Kit (DIP100, R&D Systems, Minnesota, USA) and human CXCL11/I‐TAC Quantikine ELISA Kit (DCX110, R&D Systems, Minnesota, USA), respectively, following the manufacturer's instructions.
Tang L., Zhang H., Zhou F., Wei Q., Du M., Wu J., Li C., Luo W., Zhou J., Wang X., Chen Z., Zhang Y., Huang Z., Wu Z., Wen Y., Jiang H., Liao D., Kou H., Xiong W., Mei H, & Hu Y. (2024). Targeting autophagy overcomes cancer‐intrinsic resistance to CAR‐T immunotherapy in B‐cell malignancies. Cancer Communications, 44(3), 408-432.
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2

Cytokine Profiling in Healthy, MBL, and CLL

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In 24 healthy subjects, 40 MBL and 44 CLL cases, serum levels of the following 20 cytokines were quantified: IL1β, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL15, IL17A, IFNα, IFNγ, TNFα, GM-CSF, CCL3, CCL4, CCL19, CXCL10 and CXCL11 using the U-PLEX Platform (Meso Scale Discovery, Rockville, MD, USA) and CXCL9 using Human CXCL9/MIG Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA). All determinations were performed in duplicate and concentrations were reported in picograms per milliliter. To study the potential cytokine interactions, their respective coding genes were obtained and genomic data was analyzed using IPA.
Blanco G., Puiggros A., Sherry B., Nonell L., Calvo X., Puigdecanet E., Chiu P.Y., Kieso Y., Ferrer G., Palacios F., Arnal M., Rodríguez-Rivera M., Gimeno E., Abella E., Rai K.R., Abrisqueta P., Bosch F., Calon A., Ferrer A., Chiorazzi N, & Espinet B. (2021). CLL-like monoclonal B cell lymphocytosis displays an increased inflammatory signature that is reduced in early stage chronic lymphocytic leukemia. Experimental hematology, 95, 68-80.
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3

Quantifying CXCL9 in Stimulated HUVECs

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After stimulation with IL-1β, the HUVEC condition medium was collected at the time point of 18 h and 24 h. Quantitation of CXCL9 protein expression was performed using Human CXCL9/MIG Quantikine ELISA Kit (R&D systems, USA). The results were detected at a wavelength of 450 nm using multimode microplate readers (Infinite 200, TECAN).
Guo Y.C., Chiu Y.H., Chen C.P, & Wang H.S. (2018). Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration. Stem Cell Research & Therapy, 9, 281.
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4

Quantitative ELISA for Chemokines

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Concentrations of chemokines in the supernatant were measured with sandwich ELISA kits, human CXCL9/MIG Quantikine ELISA Kit and human CXCL10/IP-10 Quantikine ELISA Kit (R&D Systems), according to the manufacturer's instructions.
Nakayama T., Yoshimura M., Higashioka K., Miyawaki K., Ota Y., Ayano M., Kimoto Y., Mitoma H., Ono N., Arinobu Y., Kikukawa M., Yamada H., Akashi K., Horiuchi T, & Niiro H. (2021). Type 1 helper T cells generate CXCL9/10-producing T-bet+ effector B cells potentially involved in the pathogenesis of rheumatoid arthritis. Cellular immunology, 360.
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