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BNIP3L is a lab equipment product offered by Cell Signaling Technology. It is a protein that plays a role in the regulation of cellular processes. The core function of BNIP3L is to facilitate the degradation of mitochondria through a process called mitophagy.

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Lab products found in correlation

Vdac1, by Abcam (1 mentions) Ubiquitin, by PTM Biolabs (1 mentions) Pink1, by Abcam (1 mentions) Ciap2, by Cell Signaling Technology (1 mentions) Cleaved caspase 3 and 8, by Cell Signaling Technology (1 mentions) C flip, by Cell Signaling Technology (1 mentions) Caspase 9, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Mcl 1, by Cell Signaling Technology (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

BNIP3L/Nix (3 citations) Anti-BNIP3L (2 citations)

2 protocols using bnip3l

1

Protein Extraction and Western Blot Analysis

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The methods used for protein extraction from in vitro and in vivo samples and for western blot analysis have been described previously (Yu et al., 2017 (link)). The antibodies used include BNP (Abcam, ab236101, 1:500), MHC (Abcam, ab180779, 1:1000), Vinculin (Abcam, ab129002, 1:500), p62 (Cell Signaling Technology, #5114, 1:1000), LC3 (Abcam, ab48394, 1:500), β-actin-HRP (Kang Cheng, KC-5A08, 1:5000), VDAC1 (Abcam, ab154856, 1:1000), Ubiquitin (PTM BIO, PTM-1107, 1:2000), PINK1 (Abcam, ab23707, 1:500), Parkin (Abcam, ab77924, 1:500), FUNDC1 (Abcam, ab224722, 1:500), PHB2 (Santa Cruz, sc133094, 1:1000), BNIP3L (Cell Signaling Technology, #12396, 1:1000), p-Parkin (Ser65) (Affinity Biotech, AF3500, 1:1000).
Abudureyimu M., Yu W., Cao R.Y., Zhang Y., Liu H, & Zheng H. (2020). Berberine Promotes Cardiac Function by Upregulating PINK1/Parkin-Mediated Mitophagy in Heart Failure. Frontiers in Physiology, 11, 565751.
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2

LMB100 Cytotoxicity Mechanism Evaluation

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LMB100 (RG7787, huSS1(Fab)-LR-GGS-LO10-PE24) was supplied by Roche Innovation Center (Penzberg, Germany). Panbinostat was purchased from Selleck (Houston, TX). Antibodies detecting EF2, BAX, BAK, BID, BCLxl, MCL1, Caspase- 9 and cleaved caspase-3 and −8, PARP, CIAP2, c-FLIP, BNIP3L and GAPDH were purchased from Cell Signaling (Danvers, MA).
Liu X.F., Zhou Q., Hassan R, & Pastan I. (2017). Panbinostat decreases cFLIP and enhances killing of cancer cells by immunotoxin LMB-100 by stimulating the extrinsic apoptotic pathway. Oncotarget, 8(50), 87307-87316.
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