Scopolamine hydrobromide, 1–1-diphenyl 2-picrylhydrazyl radical scavenging method (DPPH), 5,5′-dithio-bis-2-nitrobenzoic acid (DTNB), reduced glutathione (GSH), Folin–Ciocalteau, catechin and gallic acid were purchased from Sigma-Aldrich, USA. The rat amyloid beta-42 (Aβ42) ELISA kit was purchased from CUSABIO Technology, China (Cat# CSB-E10786r). The primers of AChE, Tau, TNF-α, A disintegrin and metalloprotease-17 (ADAM-17), cAMP-response element-binding protein (CREB), brain-derived neurotropic factor (BDNF), inducible nitric oxide (iNOS) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Bioneer, Korea. Total RNA extraction kit, cDNA synthesis kit and OneTaq Quick –Load 2X Master Mix were obtained from iNtRON Biotechnology, Korea. All other analytical grade chemicals were obtained from Sigma (Germany) and Merck (Germany).
Glyceraldehyde 3 phosphate dehydrogenase gapdh
Manufactured by Bioneer
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis, the metabolic pathway that converts glucose into energy. GAPDH is responsible for the oxidation of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate.
Automatically generated - may contain errors
Lab products found in correlation
Scopolamine hydrobromide, by Merck Group (1 mentions)
Folin ciocalteau, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Total rna extraction kit, by iNtRON Biotechnology (1 mentions)
Cdna synthesis kit, by iNtRON Biotechnology (1 mentions)
Nmr solvents, by Cambridge Isotopes (1 mentions)
Pcsk9, by Abcam (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Pcsk9, by Bioneer (1 mentions)
Hnf1a, by Bioneer (1 mentions)
Srebf1, by Bioneer (1 mentions)
Srebf2, by Bioneer (1 mentions)
Chloroauric acid haucl4, by Merck Group (1 mentions)
Trap assay kit, by Takara Bio (1 mentions)
Cell culture medium, by Thermo Fisher Scientific (1 mentions)
Macrophage colony stimulating factor m csf, by Thermo Fisher Scientific (1 mentions)
Rankl, by Thermo Fisher Scientific (1 mentions)
3 protocols using glyceraldehyde 3 phosphate dehydrogenase gapdh
1
Molecular Mechanisms of Scopolamine-Induced Cognitive Impairment
2
HepG2 Cell Culture and Molecular Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HepG2 human
hepatocellular liver cell line (provided by the Korea Research Institute
of Bioscience and Biotechnology, South Korea) was cultured according
to the previous publication.48 (link) Eagle’s
minimum essential medium, penicillin, streptomycin of Hyclone (Logan,
UT, USA), and bovine serum albumin from Sigma-Aldrich (St. Louis,
MO, USA) were used for cell culture. Antibodies (PCSK9, LDLR, and
β-actin) were purchased from Abcam, Inc. (Cambridge, MA, USA).
Primers for PCSK9, LDLR, HNF1a, SREBF1, SREBF2, and glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) were purchased from Bioneer Corp. (Daejeon,
South Korea). Solvents for extraction and isolation (ethanol, methanol,
chloroform, etc.) were provided by SK Chemicals (Seoul, Korea). HPLC-grade
solvents, acetonitrile and methanol (SK Chemicals, Seoul, South Korea),
were used for HPLC. NMR solvents were purchased from Cambridge Isotope
Laboratories, Inc. (Andover, MA, USA).
hepatocellular liver cell line (provided by the Korea Research Institute
of Bioscience and Biotechnology, South Korea) was cultured according
to the previous publication.48 (link) Eagle’s
minimum essential medium, penicillin, streptomycin of Hyclone (Logan,
UT, USA), and bovine serum albumin from Sigma-Aldrich (St. Louis,
MO, USA) were used for cell culture. Antibodies (PCSK9, LDLR, and
β-actin) were purchased from Abcam, Inc. (Cambridge, MA, USA).
Primers for PCSK9, LDLR, HNF1a, SREBF1, SREBF2, and glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) were purchased from Bioneer Corp. (Daejeon,
South Korea). Solvents for extraction and isolation (ethanol, methanol,
chloroform, etc.) were provided by SK Chemicals (Seoul, Korea). HPLC-grade
solvents, acetonitrile and methanol (SK Chemicals, Seoul, South Korea),
were used for HPLC. NMR solvents were purchased from Cambridge Isotope
Laboratories, Inc. (Andover, MA, USA).
3
Osteoclast Differentiation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chloroauric acid (HAuCl4) and trisodium citrate were purchased from Sigma Aldrich. (St. Louis, MO, USA), and 30 nm SNPs were purchased from Cytodiagnostics (Burlington; ON, Canada). The cell culture medium was purchased from GIBCO (Grand Island, NY, USA). Macrophage-colony stimulating factor (M-CSF) and RANKL were purchased from Peprotech (London, UK). The TRAP assay kit used here was purchased from Takara (Shiga, Japan). EZ-Cytox was purchased from Dogen (Seoul, Korea). All primers for Cathepsin K (CTSK), c-FOS, the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), TRAP, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Bioneer (Seoul, Korea). The antibodies used in this study were purchased from Invitrogen (Carlsbad, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!