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Caspase 9 antibodies

Manufactured by BD

Caspase-9 antibodies are laboratory reagents used to detect and measure the presence of the Caspase-9 protein, which plays a key role in the apoptosis (programmed cell death) process. These antibodies can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and activation of Caspase-9 in biological samples.

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3 protocols using caspase 9 antibodies

1

Pristimerin-Induced Apoptosis in Cancer Cells

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Pristimerin was purchased from Sigma Chemicals (St. Louis, MO). Anti-caspase-3 and caspase-9 antibodies were purchased from BD Pharmingen (San Diego, CA). Anti-PARP-1, anti-Bcl-2, anti-Bcl-xL and anti-survivin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). 96 AQueous One Solution Proliferation Assay System was from Promega (Madison, WI). Annexin V-FITC apoptosis detection kit was purchased from BD Pharmingen and mitochondrial potential sensor JC-1 was obtained from Molecular Probes, Invitrogen (San Diego, CA). Caspase-3 activity kit was from Clontech Laboratories (Mountain View, CA). Stock solution of PM (100 mM) was prepared in DMSO and all test concentrations were prepared by diluting stock solution in tissue culture medium.
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2

Investigating Cellular Stress Pathways

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Whole cell extracts in RIPA buffer with proteasome inhibitors were isolated for SDS-PAGE and used for the western blot analysis. Anti- p53, caspase-7, and caspase-9 antibodies were ordered from BD Pharmingen (San Diego, CA). Antibodies against total and phosphorylated H2AX, p21, and GAPDH were purchased from Abcam Inc. (Cambridge, MA). Antibodies against PARP and GSTP1 were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-LC3 polyclonal antibody was ordered from Novus Biological Inc. (Littleton, CO).
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3

Investigating Cellular Stress Pathways

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Whole cell extracts in RIPA buffer with proteasome inhibitors were isolated for SDS-PAGE and used for the western blot analysis. Anti- p53, caspase-7, and caspase-9 antibodies were ordered from BD Pharmingen (San Diego, CA). Antibodies against total and phosphorylated H2AX, p21, and GAPDH were purchased from Abcam Inc. (Cambridge, MA). Antibodies against PARP and GSTP1 were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-LC3 polyclonal antibody was ordered from Novus Biological Inc. (Littleton, CO).
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