Fourier transform infrared (FTIR) spectra were obtained on a Bruker Vector 22 spectrophotometer (Shimadzu Corp., Kyoto, Japan) in the range of 4,000−500 cm−1 and recorded on solid samples in a KBr matrix. Fluorescence spectra were obtained on an RF-5301 spectrofluorometer (Shimadzu Corp.) equipped with a 450 W Xenon lamp. Ultraviolet (UV)–visible absorption spectra were measured using UV–visible spectrometer (Lambda 35; PerkinElmer Inc., Waltham, MA, USA). X-ray photoelectron spectroscopy (XPS) was obtained on an axis ultra-spectrometer (Kratos, Manchester, UK) by using mono-Al K a line (1,486.71 eV) radiation at a power of 225 W. Dynamic light scattering (DLS; Zetasizer Nano ZS-90; Malvern Instruments, Malvern, UK) and TEM (Tecnai G2 20; FEI, Hillsboro, OR, USA) were used for characterizing particle size, zeta potential, and morphology of Gd-CDs and Gd-CDs/AFn (DOX)/FA, respectively. The in vitro and in vivo T1-weighted MR images were conducted on a 3 T clinical MRI scanner (Siemens, Munich, Germany).
3 t clinical mri scanner
Manufactured by Siemens
Sourced in Germany
The 3 T clinical MRI scanner is a diagnostic imaging device that uses a strong magnetic field and radio waves to produce high-resolution images of the body's internal structures. It is designed to operate at a magnetic field strength of 3 Tesla, which provides enhanced image quality and detail compared to lower field strength MRI scanners. The 3 T clinical MRI scanner is a versatile tool used in various medical applications, including neuroimaging, musculoskeletal imaging, and body imaging.
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Lab products found in correlation
Axis ultra spectrometer, by Shimadzu (1 mentions)
Tecnai g2 20, by Thermo Fisher Scientific (1 mentions)
Lambda 35, by PerkinElmer (1 mentions)
Rf 5301 spectrofluorometer, by Shimadzu (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Magnevist, by Bayer (1 mentions)
Diltiazem, by Merck Group (1 mentions)
Mncl2, by Merck Group (1 mentions)
4 protocols using 3 t clinical mri scanner
1
Characterization of Gadolinium-Doped Carbon Dots
2
3T MRI Brain Protocol for Tumor Analysis
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A routine brain MRI protocol, which included pre- and post-contrast T1-weighted MRI (T1WI and ce-T1WI, respectively) (repetition time [TR]/echo time [TE] = 589/10 ms, field of view [FOV] = 220 mm, slice thickness = 3 mm), T2-weighted MRI (T2WI) (TR/TE = 3810/101 ms, FOV = 220 mm, slice thickness = 3 mm), fluid-attenuated inversion recovery (FLAIR) (TR/TE = 8320/92 ms, TI = 2000 ms, FOV = 220 mm, slice thickness = 3 mm), and SWI-MRI (TR/TE = 28/20 ms, FOV = 220 mm, slice thickness = 1.6 mm), was performed using a 3T clinical MRI scanner (Siemens Healthcare, Erlangen, Germany). After completing the pre-contrast series, a manual injection of 0.2 mL per kilogram of Gd-DTPA (Magnevist, Bayer HealthCare Pharmaceuticals, Wayne, NJ, USA) was administered to the patient, after which the post-contrast series was acquired. The dosage range for this injection varied from 7.5 mL to 15 mL depending on the patient’s weight. The MRI scanner software (Syngo XA30) automatically generated the magnitude and phase images, which were combined to obtain the post-processed SWI image. Subsequently, the phase, magnitude, and post-processed SWI images were recorded. A senior radiologist with 31 years of experience (A.D) reviewed all the MRI data, confirming that the images were acquired correctly, encompassed the tumor region, and exhibited a signal-to-noise ratio suitable for quantitative analysis.
3
Manganese-Enhanced MRI of NSCs in TBI
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As mentioned above, approximately 1 month after transplantation of NSCs into the TBI rat brains, SPIOs-labeled NSCs could migrate to the injured brain areas. Then, manganese-enhanced MRI (ME-MRI) scan was performed to detect the function of these NSCs16 (link).
In brief, 1% MnCl2 (Sigma-Aldrich) was first intravenously infused into group A TBI rats within 1 h. At approximately the halfway stage of MnCl2 infusion, blood–brain barrier (BBB) of the right-side cerebral hemisphere (the injury site) was opened by 20% mannitol. Then, left (contralateral) forepaw electrical stimulation was conducted for 30 min, and the ME-MRI scan was then performed. In group B TBI rats, the same procedures were performed, but the Ca2+ channel inhibitor diltiazem (Sigma-Aldrich) was infused 10 min before electrical stimulation and was continued during the entire stimulus period.
Next, ME-MRI scan was performed using a clinical 3 T MRI scanner (Siemens) with an animal coil and the three-dimensional spoiled gradient recalled acquisition in a steady state (3D-SPGR) pulse sequence was used. The scan was performed using the following parameters: TE = 2.4 ms; TR = 8.8 ms; FOV = 5 cm × 4 cm; flip angle = 45°; repetition = 6 NEX.
In brief, 1% MnCl2 (Sigma-Aldrich) was first intravenously infused into group A TBI rats within 1 h. At approximately the halfway stage of MnCl2 infusion, blood–brain barrier (BBB) of the right-side cerebral hemisphere (the injury site) was opened by 20% mannitol. Then, left (contralateral) forepaw electrical stimulation was conducted for 30 min, and the ME-MRI scan was then performed. In group B TBI rats, the same procedures were performed, but the Ca2+ channel inhibitor diltiazem (Sigma-Aldrich) was infused 10 min before electrical stimulation and was continued during the entire stimulus period.
Next, ME-MRI scan was performed using a clinical 3 T MRI scanner (Siemens) with an animal coil and the three-dimensional spoiled gradient recalled acquisition in a steady state (3D-SPGR) pulse sequence was used. The scan was performed using the following parameters: TE = 2.4 ms; TR = 8.8 ms; FOV = 5 cm × 4 cm; flip angle = 45°; repetition = 6 NEX.
4
Magnetic Field Characterization of iPS-derived NSCs
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To evaluate the magnetic field difference of iPS cells-derived NSCs (SPIOs-labeled vs. Unlabeled), two Eppendorf tubes (250 µl) of cell suspension (SPIOs-labeled vs. Unlabeled) were prepared. Two control Eppendorf tubes were SPIOs solution and cell medium. The four Eppendorf tubes were then sent for MRI scan using the clinical 3 T MRI scanner (Siemens, Munich, Germany) with an animal coil. T2*-weighted MRI images were acquired with the following parameters: repetition time (TR) = 475 ms, echo time (TE) = 20 ms, field of view (FOV) = 80 mm × 100 mm, matrix = 260 × 320, slice thickness = 2 mm.
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