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Xtt based viability assay

Manufactured by Sartorius
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Sourced in Israel

The XTT-based viability assay is a colorimetric method used to measure the metabolic activity of cells. It utilizes the XTT tetrazolium salt, which is reduced by active cells to produce a colored formazan product. The intensity of the color change is directly proportional to the number of viable cells, allowing for the quantification of cell viability.

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XTT assay

2 protocols using xtt based viability assay

1

Cell Viability Assay for MicroNB

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MicroNB-shControl and MicroNB-shPHOX2B cells were seeded (1×104 per well) in a 96-well, flat-bottomed, tissue culture plate. Viability under normal growth conditions [17 (link)] was monitored in triplicates using an XTT-based viability assay after 0, 24, 48, and 72 hours, according to the manufacturer's instructions (Biological Industries). Cell viability was determined as previously described [18 (link)].
Naftali O., Maman S., Meshel T., Sagi-Assif O., Ginat R, & Witz I.P. (2016). PHOX2B is a suppressor of neuroblastoma metastasis. Oncotarget, 7(9), 10627-10637.
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2

Neuroblastoma Cell Viability Assay

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Neuroblastoma cells were seeded (1 × 105 per well) in triplicate in a 96-well tissue culture plate. Viability was monitored using XTT-based viability assay according to the manufacturer's instructions (Biological Industries, Beit Haemek, Israel).
Edry Botzer L., Maman S., Sagi-Assif O., Meshel T., Nevo I., Yron I, & Witz I.P. (2016). Hexokinase 2 is a determinant of neuroblastoma metastasis. British Journal of Cancer, 114(7), 759-766.
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