To study nuclear phenotypes, cells were fixed for 15 min on ice with 1/10 (v/v) glutaraldehyde and washed thrice with ice cold 1 × PBS before mounting on slides with an equal volume of 100 µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) and observed using a Nikon Eclipse Ti-E inverted microscope using Plan-Apo 100× objective lens under oil immersion (Nikon, Tokyo, Japan). Image acquisition was performed with Nikon NIS-Elements AR software.
Plan apo 100 objective lens
Manufactured by Nikon
Sourced in Japan
The Plan-Apo 100× objective lens is a high-magnification lens designed for use in microscopy applications. It features a high numerical aperture and is optimized for flat-field imaging, providing a wide, flat field of view. The lens is constructed with premium optical materials and coatings to deliver exceptional image quality and resolution.
Automatically generated - may contain errors
2 protocols using plan apo 100 objective lens
1
Nuclear Phenotyping with DAPI Staining
2
Nuclear Phenotyping of Log-Phase Cells
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For nuclear phenotype observation, log-phase cells were fixed with 1/10 (v/v) glutaraldehyde on ice for 15 min, and then rinsed thrice with cold 1 × PBS before mounting onto glass slides with an equal volume of 100 μg/ml DAPI stain (Sigma-Aldrich). For direct observation of GFP signals, log-phase cells were concentrated by filtration through GF/C glass microfiber filters (GE Healthcare, Little Chalfont, UK) and then fixed for 2 h in 100% methanol at −80°C. Cells were progressively rehydrated before mounting with 1 μg/ml DAPI for microscopy with a Nikon Eclipse Ti-E inverted microscope using Plan-Apo 100 × objective lens with oil immersion (Nikon, Tokyo, Japan). Image intensities were measured using the Nikon NIS-Elements AR software and ImageJ.
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