Advanced DMEM/F12, Glutamax and B27 and N2 were purchased from InVitrogen. Mouse colonic crypt culture; murine recombinant epidermal growth factor and noggin from Peprotech, mouse recombinant R-spondin 1 from R & D Systems. Human recombinant growth factors used for human colonic crypt culture experiments were: insulin growth factor-1 (Sigma); R-spondin 1 (Sino Biological or R&D Systems); Wnt 3A (R&D systems); noggin (Peprotech). Growth factor-reduced Matrigel was purchased from VWR International.
Insulin growth factor 1
Manufactured by Merck Group
Insulin growth factor-1 (IGF-1) is a protein that plays a key role in cell growth and development. It is a laboratory reagent used for research purposes in various fields, including cell biology, endocrinology, and developmental biology. IGF-1 is a potent stimulator of cell proliferation and differentiation, and it is involved in the regulation of various physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
R spondin 1, by Sino Biological (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Mouse recombinant r spondin 1, by R&D Systems (2 mentions)
Advanced dmem f12, by Thermo Fisher Scientific (2 mentions)
Noggin, by Thermo Fisher Scientific (2 mentions)
Wnt3a, by R&D Systems (2 mentions)
Murine recombinant epidermal growth factor, by Thermo Fisher Scientific (1 mentions)
Growth factor reduced matrigel, by Avantor (1 mentions)
2 protocols using insulin growth factor 1
1
Murine and Human Colonic Crypt Culture
2
Intestinal Organoid Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Advanced DMEM/F12, GlutaMAX, B27, and N2 were purchased from Invitrogen. Mouse recombinant growth factors used for mouse colonic crypt culture; murine recombinant epidermal growth factor and Noggin (PeproTech), mouse recombinant R-spondin 1 (R&D Systems). Human recombinant growth factors used for human colonic crypt culture experiments were insulin growth factor-1 (Sigma), R-spondin 1 (Sino Biological or R&D Systems), Wnt 3A (R&D Systems), and Noggin (PeproTech). Matrigel Matrix, Growth-Factor Reduced was purchased from VWR International.
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