Globulin-free HSA (A3782), warfarin (A2250), ibuprofen (I4883) and hemin (51,280) were purchased from Sigma-Aldrich (St. Louis, MO). Spectroscopic grade DMSO was also purchased from Sigma-Aldrich (St. Louis, MO) while TSPP was obtained from Frontier Scientific (Logan, UT). All chemicals were used without further purification.
Ibuprofen i4883
Manufactured by Merck Group
Ibuprofen (I4883) is a laboratory reagent produced by Merck Group. It is a non-steroidal anti-inflammatory drug (NSAID) that can be used in research applications. The product is supplied as a crystalline powder.
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Lab products found in correlation
3 protocols using ibuprofen i4883
1
Purification and Characterization of Biomolecules
2
Evaluating NSAID Cytotoxicity in Cell Cultures
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ibuprofen (I4883) and meloxicam (M3935) were purchased from Sigma-Aldrich. For cell culture experiments, ibuprofen and meloxicam were solubilized in DMSO at a stock concentration of 10 mM and then diluted in media to make working solutions. For mouse experiments, stock solutions of ibuprofen (300 mg/ml) and meloxicam (10 mg/ml) were prepared in DMSO and then diluted 100-fold in PBS to make working solutions. To determine the maximum non-toxic dose of NSAIDs to use for cell culture experiments, cells were treated with different concentrations of ibuprofen or meloxicam for 48 hours, and cell viability was measured by CellTiter-Glo® Luminescent Cell Viability Assay (Promega) following manufacturer’s instructions.
3
Determining Optimal NSAID Concentrations
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ibuprofen (I4883) and meloxicam (M3935) were purchased from Sigma-Aldrich. For cell culture experiments, ibuprofen and meloxicam were solubilized in DMSO at a stock concentration of 10 mM and then diluted in medium to make working solutions. For mouse experiments, stock solutions of ibuprofen (300 mg/ml) and meloxicam (10 mg/ml) were prepared in DMSO and then diluted 100-fold in phosphate-buffered saline (PBS) to make working solutions. To determine the maximum nontoxic dose of NSAIDs to use for cell culture experiments, cells were treated with different concentrations of ibuprofen or meloxicam for 48 h, and cell viability was measured by a CellTiter-Glo luminescent cell viability assay (Promega) according to the manufacturer’s instructions.
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