N tert butyldimethylsilyl n methyltrifluoroacetamide

Leaves were sampled
at the postflowering stage. For each sample, there were three technical
replicates. The air-dried Brassica leaves were pulverized in a mortar
and pestle into a fine powder. One gram powder of each sample was
extracted three times, with 5 mL of methanol at room temperature,
and sonicated for 15 min and centrifuged. The extract was concentrated
to 1 mL by vacuo. After centrifugation, the solvent portion was collected
and filtered using syringe filters (0.45 μm) and dissolved in N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (Alfa Aesar, Fisher scientific, USA). Samples
were then immediately analyzed using GC-MS for untargeted metabolites.^{33 (link)}