Th 01

Manufactured by Omni International

The TH-01 is a laboratory equipment designed for temperature and humidity monitoring. It is capable of measuring and displaying both temperature and relative humidity values simultaneously. The device features a digital display and provides accurate readings within a specified range.

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Lab products found in correlation

Pierce ripa lysis buffer, by Thermo Fisher Scientific (1 mentions) West pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Immobilon p membrane, by Merck Group (1 mentions) Cdcp1, by Cell Signaling Technology (1 mentions) Nw04120box, by Thermo Fisher Scientific (1 mentions) Halt protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions)

2 protocols using th 01

Subcellular Fractionation of Diaphragm Muscle

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Fresh diaphragm muscle tissue was rinsed with cold PBS and then homogenized in homogenization buffer (250 mM sucrose, 50 mM Tris-HCL pH7.4, 5 mM MgCl2, supplemented with protease and phosphatase inhibitors cocktail) with a homogenizer (Omni International, TH-01). After incubation on ice for 30 min, the homogenates were centrifuged at 800 g for 15 min to separate the crude supernatant and pellet. This crude supernatant was further centrifuged at 14,000 g for 10 min to further remove the insoluble cell debris. The final supernatant from this centrifugation was saved as the cytoplasmic fraction. The crude pellet was re-suspended with the above homogenization buffer and centrifuged at 800 g for 15 min. After 3 repeats of this cycle of resuspension and centrifugation, the washed pellets were lysed with RIPA buffer. After 14, 000 g centrifugation, the supernatant of the lysate was saved as nuclear fraction.

Tang H., L. Kennedy C., Lee M., Gao Y., Xia H., Olguin F., Fraga D.A., Ayers K., Choi S., Kim M., Tehrani A., Sowb Y.A., Rando T.A, & Shrager J.B. (2017). Smad3 initiates oxidative stress and proteolysis that underlies diaphragm dysfunction during mechanical ventilation. Scientific Reports, 7, 14530.

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Western Blot Analysis of Tumor Samples

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Tumor samples were added to Pierce RIPA lysis buffer (89900, ThermoFisher Scientific) with Halt protease and phosphatase inhibitors (1861281, ThermoFisher Scientific). The samples were homogenized using a probe sonicator (Omni TH-01) and then centrifuged for 15 minutes at 15,000 ×g. 4X SDS-loading buffer was added to protein lysates and 15 μg of lysate were resolved on a 4–12% Bis Tris gel (NW04120BOX, Invitrogen). Gels were transferred onto an Immobilon-P membrane (IPVH00010, Millipore). Membranes were blocked in 5% milk in TBST for 90 minutes before being placed in a primary antibody solution. Primary antibodies used were CDCP1 (4115, Cell Signaling, 1:1000), PSMA (12815, Cell Signaling, 1:1000), and B-Actin (A5441, Sigma-Aldrich, 1:5000). Membranes were then washed and incubated with a secondary antibody solution for 30 min at room temperature. Secondary antibodies used were goat anti-rabbit (65-6120, Invitrogen, 1:5000) and goat anti-rat (62-6520, Invitrogen, 1:5000). Proteins were detected using West Pico Chemiluminescent Substrate for 20 seconds (34578, ThermoFisher Scientific) and then exposed to film (30-507, Blue Devil). Each immunoblot was reproduced at least once with freshly harvested protein samples.

Zhao N., Chopra S., Trepka K., Wang Y.H., Sakhamuri S., Hooshdaran N., Kim H., Zhuo J., Lim S.A., Leung K.K., Egusa E.A., Zhu J., Zhang L., Foye A., Sriram R., Chan E., Seo Y., Feng F.Y., Small E.J., Chou J., Wells J.A., Aggarwal R, & Evans M.J. (2022). CUB domain containing protein 1 (CDCP1) is a target for radioligand therapy in castration resistant prostate cancer including PSMA null disease. Clinical cancer research : an official journal of the American Association for Cancer Research, 28(14), 3066-3075.

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