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Caspase 1 fluorometric kit

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The Caspase-1 fluorometric kit is a tool for measuring the activity of the caspase-1 enzyme. Caspase-1 is an important protease involved in the inflammatory response and apoptosis. The kit provides a sensitive and quantitative method to detect caspase-1 activity in cell and tissue samples.

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Caspase-1 (146 citations) Caspase-1 Fluorometric Assay Kit (15 citations) Caspase–1/ICE Fluorometric Assay kit (4 citations)

2 protocols using caspase 1 fluorometric kit

1

Caspase-1 Activity Quantification

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Caspase-1 activity was determined using caspase-1 fluorometric kit (Cat# K110) (Bio Vision, Milpitas, CA) or Caspase-Glo® 1 homogeneous luminescent assay (Cat# G9951)(Promega Corporation, Madison, WI) kit as per manufacturer’s protocol. Change in fluorescence or luminescence (depending on the kit) was plotted as relative fluorescence units (RFU) or relative luminescence units (RLU) respectively.
Singh D.P., Kaur G., Bagam P., Pinkston R, & Batra S. (2018). Membrane microdomains regulate NLRP10 and NLRP12 dependent signalling in A549 cells challenged with Cigarette Smoke Extract. Archives of toxicology, 92(5), 1767-1783.
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2

Caspase-1 Activity Assay in Adipose Cells

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The caspase-1 activity in the lysates of SVF, 3T3-L1 preadipocytes and adipose tissue preadipocytes was determined using a caspase-1 fluorometric kit (Biovision, San Francisco, CA, USA) as previously described [44 (link)]. Cells were lysed in hypotonic cell lysis buffer on ice for 10 min and centrifuged to remove the insoluble fraction (12,000× g, 10 min). A total of 50 μL SVF lysate was incubated with 50 μM peptide YVAD-AFC and reaction buffer (50 μL) for 2 h. The fluorescence of the cleaved substrate was measured using a fluorometer at set time intervals.
Zhu P., Zhang J.J., Cen Y., Yang Y., Wang F., Gu K.P., Yang H.T., Wang Y.Z, & Zou Z.Q. (2022). High Endogenously Synthesized N-3 Polyunsaturated Fatty Acids in Fat-1 Mice Attenuate High-Fat Diet-Induced Insulin Resistance by Inhibiting NLRP3 Inflammasome Activation via Akt/GSK-3β/TXNIP Pathway. Molecules, 27(19), 6384.
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