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Ab32457

Manufactured by Cedarlane
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The Ab32457 is a piece of laboratory equipment designed for general scientific applications. It functions as a tool for conducting research and analysis in a controlled laboratory setting. The core purpose of this product is to assist with various experimental procedures, but no further details on its intended use can be provided in an unbiased and factual manner.

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Lab products found in correlation

Prolong gold antifade, by Thermo Fisher Scientific (2 mentions) A21206, by Abcam (2 mentions) Cl8942ap, by Cedarlane (2 mentions) D3571, by Thermo Fisher Scientific (2 mentions)

2 protocols using ab32457

1

Multicolor Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
After blocking, slides were incubated with the following primary antibodies: CD31 (Abcam ab32457, 1:100), LGALS3 (Cedarlane CL8942AP, 1:500) or their isotype IgG controls. Each slide had at least two sections stained with primary antibody and at least one section used as an IgG control. ACTA2 was conjugated to -FITC (Sigma F3777 clone 1A4 1:500). Secondary antibodies for immunofluorescence were as follows: donkey anti-rabbit 555 (Invitrogen A21206, 1:250) and donkey anti-rat Dylight 650 (Abcam ab102263, 1:250). Nuclear counterstain was performed with DAPI (ThermoFisher Scientific D3571), and slides were mounted with Prolong Gold Antifade (Invitrogen).
Newman A.A., Serbulea V., Baylis R.A., Shankman L.S., Bradley X., Alencar G.F., Owsiany K., Deaton R.A., Karnewar S., Shamsuzzaman S., Salamon A., Reddy M.S., Guo L., Finn A., Virmani R., Cherepanova O.A, & Owens G.K. (2021). Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms. Nature metabolism, 3(2), 166-181.
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2

Multicolor Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
After blocking, slides were incubated with the following primary antibodies: CD31 (Abcam ab32457, 1:100), LGALS3 (Cedarlane CL8942AP, 1:500) or their isotype IgG controls. Each slide had at least two sections stained with primary antibody and at least one section used as an IgG control. ACTA2 was conjugated to -FITC (Sigma F3777 clone 1A4 1:500). Secondary antibodies for immunofluorescence were as follows: donkey anti-rabbit 555 (Invitrogen A21206, 1:250) and donkey anti-rat Dylight 650 (Abcam ab102263, 1:250). Nuclear counterstain was performed with DAPI (ThermoFisher Scientific D3571), and slides were mounted with Prolong Gold Antifade (Invitrogen).
Newman A.A., Serbulea V., Baylis R.A., Shankman L.S., Bradley X., Alencar G.F., Owsiany K., Deaton R.A., Karnewar S., Shamsuzzaman S., Salamon A., Reddy M.S., Guo L., Finn A., Virmani R., Cherepanova O.A, & Owens G.K. (2021). Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms. Nature metabolism, 3(2), 166-181.
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