Alpha amylase from aspergillus oryzae

Potassium iodate (KI), iodine (I₂), potassium hydroxide (KOH), sodium hydroxide (NaOH) and hydrochloric acid (HCl), were purchased from Merck (Darmstadt, Germany). Sodium chloride (NaCl) was purchased from BDH Chemicals (Poole, UK). Potassium chloride (KCl) was purchased from Fisher Scientific (Norcross, GA, USA). Sodium bicarbonate (NaHCO₃) was purchased from (Riedel-de Haën, Seelze, Germany). Heat stable α-amylase from Bacillus licheniformis (3000 U/mL), amyloglucosidase from Aspergillus niger (3300 U/mL), glucose oxidase peroxidase (GOPOD) kit and D-glucose standard (1 mg/mL) were purchased from Megazyme (Wicklow, Ireland). Alpha amylase from Aspergillus oryzae (30 U/mg) and pancreatin from porcine pancreas (4 × USP) were purchased from Sigma (St. Louis, MO, USA). Pepsin was purchased from PanReac AppliChem (Barcelona, Spain). Porcine bile extract was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

The chemicals such as 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), and L-Ascorbic acid were obtained from Sigma-Aldrich Co. LLC, Mumbai, India. Methanol and dimethyl sulphoxide (DMSO) were procured from Loba Chemie Pvt. Ltd., Mumbai, India. Alpha-amylase from Aspergillus oryzae and dinitrosalicylic acid (DNS) were purchased from Sigma-Aldrich Co. LLC, Mumbai. Other chemicals and reagents were of analytical grade, and the water used was double distilled. The media such as yeast peptone dextrose agar (YPDA) and yeast peptone dextrose broth (YPDB) were obtained from Himedia Laboratories Pvt. Ltd., Mumbai, India.