Log In Sign up
The largest database of trusted experimental protocols

Mir 375

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The MiR-375 is a microRNA assay product from Thermo Fisher Scientific. It is designed to detect and quantify the expression of the microRNA-375 (miR-375) in biological samples. The MiR-375 assay provides a tool for researchers to study the role and expression of this specific microRNA.

Automatically generated - may contain errors

Lab products found in correlation

Mir 21, by Thermo Fisher Scientific (1 mentions) Mir 204, by Thermo Fisher Scientific (1 mentions) Cenpf, by Thermo Fisher Scientific (1 mentions) Taqman microrna assay, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Abi 7500 real time pcr system, by Thermo Fisher Scientific (1 mentions)

4 protocols using mir 375

1

Urine Exosomal miRNA Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated by Trizol, as previously described [4 (link)]. The miRNA expression was determined by RT-PCR, according to the manufacturer's instructions and as previously described [4 (link)]. We selected miR-21, miR-204 and miR-375 (Life Technologies, Grand Island, MY, USA; miR-21 #000397, miR-204 #000508 and miR-375 #000564), which were described to be present in the urine EVs.
Wachalska M., Koppers-Lalic D., van Eijndhoven M., Pegtel M., Geldof A.A., Lipinska A.D., van Moorselaar R.J, & Bijnsdorp I.V. (2016). Protein Complexes in Urine Interfere with Extracellular Vesicle Biomarker Studies. Journal of Circulating Biomarkers, 5, 4.
+ Open protocol
+ Expand
2

Quantification of miR-375 and Genes

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The procedure for PCR quantification was previously described (13 (link),18 (link)–20 (link)). The expression levels of miR-375 (assay ID: 000564; Applied Biosystems, Foster City, CA, USA) were analyzed by TaqMan qRT-PCR assays (TaqMan MicroRNA assays; Applied Biosystems) and RNU48 (assay ID: 001006) was used for normalization. TaqMan probes and primers for MMP-13 (assay ID: Hs00233992_m1; Applied Biosystems), CENPF (assay ID: Hs01118845_m1), KIF14 (assay ID: Hs00978236_m1) and GUSB (the internal control; assay ID: Hs00939627_ml; Applied Biosystems) were used for gene expression analysis.
Osako Y., Seki N., Kita Y., Yonemori K., Koshizuka K., Kurozumi A., Omoto I., Sasaki K., Uchikado Y., Kurahara H., Maemura K, & Natsugoe S. (2016). Regulation of MMP13 by antitumor microRNA-375 markedly inhibits cancer cell migration and invasion in esophageal squamous cell carcinoma. International Journal of Oncology, 49(6), 2255-2264.
+ Open protocol
+ Expand
3

Endometrial Cancer miRNA Expression Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
miRNA-specific qPCR assays for miR-96, miR-182, miR-141, miR-129-5p, and miR-375 (Applied Biosystems) were carried out as previously described [52 (link)] on an miRNA panel composed of endometrial endometrioid adenocarcinomas and matched adjacent non-malignant tissue. Data were normalized to RNU48 endogenous RNA control [52 (link)]. Ishikawa H cells were transfected with anti-miR96 inhibitor (Life Technology) using Lipofectamine RNAiMAX (Invitrogen). Total RNA was extracted 48 hours after transfection and miRNA and mRNA expression was measured using q-PCR. Comparisons of miRNA normalized expression values (ΔCt) employed the conventional ΔΔCt fold change method [49 (link), 50 (link)].
Yang S., Jia Y., Liu X., Winters C., Wang X., Zhang Y., Devor E.J., Hovey A.M., Reyes H.D., Xiao X., Xu Y., Dai D., Meng X., Thiel K.W., Domann F.E, & Leslie K.K. (2014). Systematic dissection of the mechanisms underlying progesterone receptor downregulation in endometrial cancer. Oncotarget, 5(20), 9783-9797.
+ Open protocol
+ Expand
4

Quantification of miR-375 and miR-205 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primers of miR-375, miR-205, and U6 synthesized by FulenGen Co., Ltd. (Guangzhou, Guangdong, China) were as follows: miR-375: (F) 5′-CAGGGTCCGAGGTATT-3′ and (R) 5′-CTGCTTTGTTCGTTCG-3′; miR-205: (F) 5′-UCCUUCAUUCCACCGGAGUCUG-3′ and (R) 5′-CAGACUCCGGUGGAAUGAAGGA-3′. The expressions of miR-375 and miR-205 were quantified by qRT-PCR using SYBR-Green assays on an ABI 7500 real-time PCR system (Applied Biosystems). The results of U6 qRT-PCR gene expression by 2−ΔΔCt method were used as the control.
Wang B., Lv K., Chen W., Zhao J., Luo J., Wu J., Li Z., Qin H., Wong T.S., Yang W., Fu Q.L, & Lei W. (2016). miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT. BioMed Research International, 2016, 9652789.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!