Hplc grade acetonitrile mecn

Manufactured by Thermo Fisher Scientific

Sourced in United States, Australia

HPLC-grade acetonitrile (MeCN) is a high-purity solvent commonly used as a mobile phase in high-performance liquid chromatography (HPLC) applications. It is a clear, colorless liquid with a characteristic odor.

Automatically generated - may contain errors

Lab products found in correlation

Glacial acetic acid, by Merck Group (2 mentions) Ammonium acetate, by Merck Group (2 mentions) Milli q water purification system, by Merck Group (2 mentions) Methanol meoh, by Thermo Fisher Scientific (2 mentions) Milli q reagent water system, by Merck Group (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Hplc grade formic acid, by Maclin Biochemical Technology (1 mentions) Acteoside, by Meilun (1 mentions) Iodoacetamide iaa, by Merck Group (1 mentions) Trypsin lys c mix mass spectrometry grade, by Promega (1 mentions) Milli q system, by Merck Group (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Petroleum ether, by Beijing Chemical Works (1 mentions) Dreamtaq dna polymerase, by Thermo Fisher Scientific (1 mentions) Dntp mix, by Thermo Fisher Scientific (1 mentions) Dichloromethane, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) Triethylamine, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Acetonitrile (2707 citations) HPLC-grade acetonitrile (752 citations) HPLC grade (157 citations) Acetonitrile (MeCN), (10 citations) HPLC-grade (MeCN) (2 citations) HPLC)-acetonitrile (2 citations)

8 protocols using hplc grade acetonitrile mecn

Reagent Procurement and Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents, but peptides, were purchased
from Sigma-Aldrich, unless stated otherwise. All peptides were commercially
obtained from Biosynthesis, unless otherwise stated. HPLC grade acetonitrile
(MeCN) was purchased from Fisher. Aqueous buffers and aqueous mobile
phases for HPLC were prepared using water purified with an Elga Purelab
Milli-Q water purification system (purified to 18.2 MΩ cm) and
filtered over 0.45 μm filters.

Ludewig H., Czekster C.M., Oueis E., Munday E.S., Arshad M., Synowsky S.A., Bent A.F, & Naismith J.H. (2018). Characterization of the Fast and Promiscuous Macrocyclase from Plant PCY1 Enables the Use of Simple Substrates. ACS Chemical Biology, 13(3), 801-811.

+ Open protocol

+ Expand

Phenylethanoid Glycoside Compound Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The authentic phenylethanoid glycoside compounds were purchased as follows: acteoside (purity 98.0%) from Dalian Meilun Biological Technology Co., Ltd. (Dalian, China); isoacteoside (purity 98.0%) from Chengdu Push Bio-Technology Co., Ltd. (Chengdu, China); and genistein (internal standard, IS) from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Martynoside and crenatoside (>98.0% purity) were isolated from AIH in our laboratory and identified by combination of NMR, HPLC, and MS. HPLC-grade acetonitrile (MeCN) and methanol were purchased from Fisher Scientific Co., Ltd. (St. Louis, MO, USA). HPLC-grade formic acid was obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China), and experimental water was purified by a Milli-Q Reagent Water System (Millipore, Burlington, MA, USA).

Zhang M., Ren X., Yue S., Zhao Q., Shao C, & Wang C. (2019). Simultaneous Quantification of Four Phenylethanoid Glycosides in Rat Plasma by UPLC-MS/MS and Its Application to a Pharmacokinetic Study of Acanthus Ilicifolius Herb. Molecules, 24(17), 3117.

+ Open protocol

+ Expand

SARS-CoV-2 Spike Protein Glycopeptide Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Trypsin/Lys-C mix mass spectrometry grade was purchased from Promega (Madison, WI, USA). O-glycoprotease (IMPa) was obtained from New England Biolabs (Ipswich, MA). HPL C grade acetonitrile (MeCN) and water were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid (FA), ammonium bicarbonate (ABC), dithiothreitol (DTT), and iodoacetamide (IAA) were purchased from Sigma Aldrich (St. Louis, MO, USA). SARS CoV-2 (2019-nCoV) Spike S1 protein variants were purchased from Sino Biologicals (US Inc., Wayne, PA, USA) (Table 1).

Onigbinde S., Reyes C.D., Fowowe M., Daramola O., Atashi M., Bennett A.I, & Mechref Y. (2023). Variations in O-Glycosylation Patterns Influence Viral Pathogenicity, Infectivity, and Transmissibility in SARS-CoV-2 Variants. Biomolecules, 13(10), 1467.

+ Open protocol

+ Expand

QuEChERS-Based Pesticide Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

WondaPak Quick, an easy, cheap, effective, rugged, and safe (QuEChERS) sodium acetate (NaAC) extraction kit; and WondaPak QuEChERS 15 mL C18/PSA/GC-e/silica gel purification tube (Shimadzu, Japan) analysis protective agent containing D-(+)-ribo-γ-lactone and D-sorbitol were purchased from Beijing Bailingwei Technology Co., Ltd. (Beijing, China). Ultrapure water was purified using a Milli-Q system (Millipore, Billerica, MA, USA); Primary secondary amine (PSA, 40–63 μm, 60 Å) was purchased from Tianjin Bona Aijer Technology Co., LtD (Tianjin, China) HPLC-grade acetonitrile (MeCN) and acetone (CP) were purchased from Fisher Chemical, USA. Analytical pure glacial acetic acid (GAA) was purchased from Chengdu Kelong Chemical Reagent Factory. A total of 136 pesticide reference solutions (100–1000 μg/mL) and pesticide reference substances (purity > 95%) were obtained from Tianjin Alta Scientific Co., Ltd. (Tianjin, China). (A Chem Tek, Tianjin, China) and Beijing Manhattan Biotechnology Co., Ltd. (Beijing, China). (Be Pure, Beijing, China). The internal standard triphenyl phosphate (1ST20129-1000A, 1000 μg/mL) was obtained from Tianjin Alta Scientific Co., Ltd. (A Chem Tek, China).

Zhang D., Gou Y., Yu X., Wang M., Yu W., Zhou J., Liu W, & Li M. (2022). Detection and Risk Assessments of Multi-Pesticides in Traditional Chinese Medicine Chuanxiong Rhizoma by LC/MS-MS and GC/MS-MS. Molecules, 27(3), 622.

+ Open protocol

+ Expand

Isolation and Purification of Ranae Oviductus

Check if the same lab product or an alternative is used in the 5 most similar protocols

The petroleum ether, guanidine hydrochloride, and ammonium sulfate analytical grade were purchased from Beijing Chemical Factory (Beijing, China). The dithiothreitol (DTT) and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The HPLC-grade acetonitrile (MeCN) and HPLC-grade methanol were purchased from Fisher (Fisher Scientific, USA). The ultrapure water was obtained from a gradient water purification system (Water Purifier, Sichuan, China).
Ranae Oviductus, bullfrog oviduct, toad oviduct and frog oviduct were provided by Jilin Province Rana Industry Association which were collected from the Changbai Mountain area in the Jilin province of China. The specific location is shown on the map in Figure 1. Ten batches of Ranae Oviductus samples were collected from different regions from the main producing area of the Changbai Mountain range. The specific collection information is shown in Table 1.

Gan Y., Xiao Y., Wang S., Guo H., Liu M., Wang Z, & Wang Y. (2019). Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC. Molecules, 24(9), 1687.

+ Open protocol

+ Expand

Synthesis and Purification of DMT-dA, DMT-dG, DMT-dC, and DMT-dT Phosphoramidites

Check if the same lab product or an alternative is used in the 5 most similar protocols

DMT-dA Bz , DMT-dG iBu , DMT-dC Bz , and DMT-dT phosphoramidites; dichloromethane; acetic anhydride; lutidine; 1-methylimidazole; and trifluoroacetic acid (TFA) were from Sigma-Aldrich (St. Louis, MO). HPLC-grade acetonitrile (MeCN) with a low water content (0.002%) was from Fisher Chemical (Waltham, MA). 5-(Ethylthio)-1Htetrazole and oxidizer reagent for phosphoramidite synthesis were from Emp Biotech (Berlin, Germany). DreamTaq DNA polymerase and dNTP mix were from Thermo Scientific (Waltham, MA). Thirty-two percent ammonia solution was from Roth (Karlsruhe, Germany). Triethylamine was from Alpha Aesar (Kandel, Germany). 5′-DMT-dA Bz -, 5′-DMT-dT-, 5′-DMT-dC Ac -, and 5′-DMT-dG iBu -CPG with a pore size of 1000 Å and loading capacity of 42-45 μmol/g were from BioAutomation (Irving, TX). The BigDye Terminator v3.1 cycle sequencing kit from Applied Biosystems (Waltham, MA) was used for sequencing.

Yantsevich A.V., Shchur V.V, & Usanov S.A. (2019). Oligonucleotide Preparation Approach for Assembly of DNA Synthons. SLAS technology, 24(6).

+ Open protocol

+ Expand

Remdesivir and GS-441524 Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols

Remdesivir (GS-5734, CAS 1809249-37-3) and GS-441524 (purity 99.77%, CAS: 1191237-69-0) were purchased from Assay Matrix (Ivanhoe North, VIC, Australia). Commercially available pooled feline microsomes (3 males, S00779) were purchased from BioIVT Baltimore Maryland USA. Pooled canine microsomes (7 males, M00201) were purchased from BD Biosciences (Woburn, MA, USA) and pooled rat microsomes (male, number of individuals not specified M9066) were purchased from Sigma (Castle Hill, NSW, Australia). Microsomes were shipped on liquid nitrogen and stored at −80 °C prior to use. β-nicotinamide adenine dinucleotide phosphate sodium (NADP), glucose-6-phosphate dehydrogenase, glucose-6-phosphate, ammonium acetate, glacial acetic acid, and magnesium chloride were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). High-performance liquid chromatography (HPLC) grade acetonitrile (MeCN) and methanol (MeOH) were purchased from Thermo Fisher Scientific (Macquarie Park, NSW, Australia). Water was purified via a Milli-Q water purification system (Merk Millipore, Burlington, MA, USA).

Coggins S.J., Kimble B., Malik R., Thompson M.F., Norris J.M, & Govendir M. (2024). Assessing in vitro stability of remdesivir (GS-5734) and conversion to GS-441524 in feline plasma and whole blood. The Veterinary Quarterly, 44(1), 1-9.

+ Open protocol

+ Expand

Quantification of Remdesivir and Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

GS-441524 (purity 99.77%, CAS: 1191237-69-0), remdesivir (GS-5734, CAS 1809249-37-3), alanine (Ala) metabolite (GS-704277; CAS 1911579-04-8), and GS-441524 triphosphate (GS-443902; CAS 2725956-16-9) were purchased from Assay Matrix (Ivanhoe North, VIC, Australia), and ammonium acetate and glacial acetic acid were purchased from Sigma Aldrich (Castle Hill, NSW, Australia). HPLC grade acetonitrile (MeCN) and methanol (MeOH) were purchased from Thermo Fisher Scientific (Macquarie Park, NSW, Australia). Water was purified via Milli-Q water purification system (Merck Millipore, Burlington, MA, USA).

Kimble B., Coggins S.J., Norris J.M., Thompson M.F, & Govendir M. (2023). Quantification of GS-441524 concentration in feline plasma using high performance liquid chromatography with fluorescence detection. The Veterinary Quarterly, 43(1), 1-9.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!