βiii tubulin

Manufactured by Proteintech

Sourced in China

βIII-Tubulin is a protein that is a member of the tubulin family, which are the building blocks of microtubules. βIII-Tubulin is primarily expressed in neuronal cells and is involved in the formation and stability of microtubules.

Automatically generated - may contain errors

Lab products found in correlation

Gsdmd, by Proteintech (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions) Gsdmd antibody, by Cell Signaling Technology (1 mentions) Anti il 1β antibody, by R&D Systems (1 mentions) Nlrp3, by Abcam (1 mentions) Caspase 1, by Abcam (1 mentions) Odyssey 9120, by LI COR (1 mentions) Psen1, by Cell Signaling Technology (1 mentions) Criterion cell, by Bio-Rad (1 mentions) Revert 700, by LI COR (1 mentions) Psd95, by Proteintech (1 mentions) Caveolin 1, by Proteintech (1 mentions) Flotillin 1, by Proteintech (1 mentions) Bace1, by Cell Signaling Technology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Neuronal nuclei (neun), by Abcam (1 mentions) Adam10, by Proteintech (1 mentions)

2 protocols using βiii tubulin

Antibody Reagents for Inflammasome Analysis

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Caspase-1 (Lot: #ab179515), NLRP3 (Lot: #ab263899), and S100 antibodies (Lot: #ab52642) were purchased from Abcam (Cambridge, England, UK). GSDMD antibody (Lot: #E9S1X) and β-actin antibody (Lot: #4967) for western blotting were purchased from Cell Signaling Technology (Massachusetts, USA). The GSDMD (Lot: #20770-1-AP) and βIII-Tubulin (Lot: #66375-1-Ig) primary antibodies for immunofluorescence were purchased from Proteintech (Wuhan, China). The NeuN primary antibody (Lot: #GB11138) was obtained from Servicebio (Wuhan, China). The rat anti-IL-18 antibody (Lot: #CZU0220031) and anti-IL-1β antibody (Lot: #YR0920081) were obtained from R&D Systems (Minneapolis, MN, USA).

Wang J., Lu S., Yuan Y., Huang L., Bian M., Yu J., Zou J., Jiang L., Meng D, & Zhang J. (2023). Inhibition of Schwann Cell Pyroptosis Promotes Nerve Regeneration in Peripheral Nerve Injury in Rats. Mediators of Inflammation, 2023, 9721375.

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Western Blot Analysis of Neurodegeneration Markers

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20μg of S1 or 5μg of lipid raft lysates were resolved on 4–15% gradient gels by Criterion Cell (Bio-Rad, Hercules, CA). Gels were transferred for 1 hour at 100V using a Criterion Blotter in an ice bath onto 0.45μm PVDF membranes. Total protein was stained (Revert 700, LI-COR Biosciences, Lincoln, NE), and imaged (LI-COR Odyssey 9120). Membranes were destained and incubated 1 hour with Intercept blocking buffer. Membranes were incubated with primary antibodies for 16 hours: GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA), H3, BACE1, PSEN1, LRP1 (Cell Signaling Technology, Danvers, MA), APP, NeuN (Abcam, Waltham, MA), Flotillin1, Flotillin2, Caveolin1, RFTN1, ADAM10, PSD95, and β III tubulin (Proteintech, Rosemead, IL). Membranes were visualized using fluorescent-conjugated secondary antibodies for image analysis by ImageJ and corrected by total protein load.

Thorwald M.A., Silva J., Head E, & Finch C.E. (2022). Amyloid futures in the expanding pathology of brain aging and dementia. Alzheimer's & dementia : the journal of the Alzheimer's Association, 19(6), 2605-2617.

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