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App n

Manufactured by Merck Group

The APP-N is a laboratory equipment product manufactured by Merck Group. It is designed to perform a specific core function, but a detailed description while maintaining an unbiased and factual approach cannot be provided. Further information is not available.

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2 protocols using app n

1

Antibody Characterization for Neuronal Imaging

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Rabbit Plk2 antibodies (#7382, ICC (immunocytochemistry) 1:200, IB (immunoblotting) 1:500) used for western blotting and immunoprecipitation and guinea pig SPAR (ICC 1:400) antibodies have been described (Lee et al., 2011 (link)). The following antibodies were purchased from the indicated suppliers and used at the indicated dilutions: APP-N (Sigma A8967, ICC 1:400, IB 1:1000); RB9023 (Thermo Scientific, ICC 1:5); M3.2 (Biolegend, ICC 1:500); 4G8 (Biolegend, ICC 1:500); APP-C (Sigma A8717, ICC 1:1000); Y188 (OriGene, ICC 1:500, IB:1:1000~1:7000); C1/6.1 (Biolegend, ICC 1:400); sAPPβ (Immuno-Biological Laboratories, IB 1:500); 6E10 (Covance, ICC 1:500, IB 1:1000); 22C11 (Millipore, MAB348, IB:1:1000); mouse anti-MAP2 (Sigma, HM-2, ICC 1:400); chicken anti-MAP2 (NeuroMAb, ICC 1:400); Plk2 C-terminal C-18 and N-terminal N-17 and H-90 (Santa Cruz, ICC 1:200, IB 1:400); GFP (Invitrogen and NeuroMAb, ICC 1:200, IB 1:1000); GluA2 (BD Pharmingen, ICC 1:50~1:100); mouse anti-PSD-95 (NeuroMAb, ICC 1:200); rabbit anti-PSD-95 (Cell Signaling, ICC 1:200); guinea pig anti-Synaptotagmin 1 (Synaptic Systems, ICC 1:500); mouse anti-VGlut1 (NeuroMAb, N28/9, ICC 1:200); rabbit anti-HA (Santa Cruz, ICC 1:100); and unlabeled donkey anti-mouse IgG (Sigma). AlexaFluor-488 and AlexaFluor-555 (Invitrogen, ICC 1:200~400) were secondary antibodies used for immunocytochemistry.
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2

Antibody Profiling for Alzheimer's Research

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The following antibodies were purchased from the indicated suppliers and used at the indicated dilutions: APP-N (Sigma A8967, ICC 1:400), Y188 (OriGene, IB:1:1000~1:7000); sAPPβ (Immuno-Biological Laboratories, IB 1:500); sAPPα (Immuno-Biological Laboratories 2B3, IB 1:100); 6E10 (Covance, IHC 1:500, IB 1:1000); Plk2 C-terminal C-18 and H-90 (Santa Cruz, IHC 1:200); synaptophysin (Sigma, IB 1:500); β-actin (Sigma, IB 1:5000); anti-mouse PSD-95 (NeuroMAb, ICC 1:200). AlexaFluor-488 and AlexaFluor-555 (Invitrogen, ICC 1:200~400) were secondary antibodies used for immunocytochemistry.
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