Mgcl2 6h2o

Mussels free or with low concentrations of toxins were obtained from culture rafts from Galicia (Spain). They were maintained overnight in tanks with aerated seawater until the start of the experiment.
Purified water for analysis and preparation of culture medium was obtained from a MilliQ-gradient system fed with an Elix Advantage-10, both from Millipore. Acetonitrile and methanol of HPLC-grade were obtained from Rathburn (Walkerburn, Scotland, UK) and Labscan (from Galiza Analitica, Vigo, Spain), respectively. For the first experiment, okadaic acid was obtained from Calbiochem (for additions) (Merck, Madrid, Spain) and from IMB-NRC, Canada (OA-1c certificate reference material). For the second experiment, OA was obtained from Alfa-Aesar (Karlsruhe, Germany) (for additions) and from CIFGA (Lugo, Spain) (reference material). NaCl, KCl, EDTA, CaCl₂ 2H₂O, MgSO₄·7H₂O and ammonium hydroxide were supplied by Merck. HEPES (H3375-250G), gentamycin sulphate, L15 (Leibovitz) medium, albumin from bovine serum and trypan blue were obtained from Sigma-Aldrich (Madrid, Spain) and MgCl₂·6H₂O and sodium cyanide from Panreac (Barcelona, Spain).

The chemical reagents of α-glucosidase from Saccharomyces cerevisiae, p-nitrophenyl glucopyranoside (pNPG), lipase type II from porcine pancreas, p-nitrophenyl butyrate (pNPB), vanillic acid, 4-aminoantipyrine, horseradish peroxidase, tyramine, MAO-A, galantamine, acetylthiocholine iodide (ATCI), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), Tris, acetylcholinesterase (AChE), levodopa (L-DOPA), and tyrosinase were acquired through Sigma-Aldrich (Madrid, Spain); clorgyline and α-kojic acid were from Cymit quimica (Barcelona, Spain); MgCl₂·6H₂O, HCl, NaCl, and potassium phosphate were from Panreac (Barcelona, Spain).

Zein (Z) from corn and Co(CH₃COO)₂·4H₂O (≥ 99%) were purchased from Sigma-Aldrich (Tres Cantos, Spain). Absolute ethanol and MgCl₂·6H₂O (≥ 99%) were purchased from Panreac (Castellar del Vallés, Barcelona, Spain). AlCl₃·6H₂O (≥ 99%), Mg(NO₃)₂·6H₂O (≥ 99%), NaOH (≥ 98%) were obtained from Fluka (Madrid, Spain) and Al(NO₃)₃·9H₂O (≥ 99%) from Merck (Darmstadt, Germany). Deionized water (resistivity of 18.2 MΩ cm) was obtained with a Maxima Ultrapure Water from Elga (Celle, Germany).