Xbridge beh 130 prep c18 column

Manufactured by Waters Corporation

Sourced in United States

The XBridge BEH 130 Prep C18 Column is a high-performance liquid chromatography (HPLC) column designed for preparative-scale separations. It features a bridged ethylene hybrid (BEH) stationary phase with a 130 Å pore size, intended for the purification of compounds.

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XBridge® Peptide BEH C18 Prep 130 Å 5 μm column (10 × 150 mm) XBridge™ BEH 130 C18 XBridge® Prep BEH C18 XBridge BEH C18 column XBridge Prep C18 column XBridge BEH C18 XBridge Prep C18 XBridge C18 column BEH C18 column Prep C18 column

2 protocols using xbridge beh 130 prep c18 column

Peptide Synthesis and Purification Protocol

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The designed peptides used in this study were synthesized using a standard 9-fluorenyl methoxy carbonyl (F-moc) solid-phase synthesis technique on an automatic peptide synthesizer (433A, Applied Biosystems, MA, USA). The synthesized peptides were purified using a reverse-phase high-performance liquid chromatography (HPLC) instrument (Waters 600, MA, USA) equipped with a preparative reverse-phase column (XBridge BEH 130 Prep C18 Column, 10 μm OBD 19 × 250 mm, Waters, MA, USA). The purities of purified peptides were over 95% as confirmed with HPLC and ESI mass spectrometry (examples of the purity checks are given in Supplementary Fig. S1).

Yang C.H., Chen Y.C., Peng S.Y., Tsai A.P., Lee T.J., Yen J.H, & Liou J.W. (2018). An engineered arginine-rich α-helical antimicrobial peptide exhibits broad-spectrum bactericidal activity against pathogenic bacteria and reduces bacterial infections in mice. Scientific Reports, 8, 14602.

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Synthesis and Purification of MMP-Degradable Peptide

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The MMP-degradable peptide (GECEE-GPQGIWGQ-EECEG, MW 1936 Da) was synthesized via solid-phase peptide synthesis methods (SPPS) using an automated microwave peptide synthesizer (Liberty Blue, CEM, Matthews, NC). Following the reaction, resin containing bound peptide was collected and washed with DMF and DCM for 3 times, respectively. The peptide was then cleaved from the resin by reacting with TFA/TIS/EDT/water (90/5/2.5/2.5) mixture with continuous shaking for 4 hrs at room temperature. Most solvent was evaporated under N₂ and the peptide was precipitated in cold ether. After centrifugation, raw peptide was re-dissolved in water and lyophilized. The peptide was purified via reverse-phase high performance liquid chromatography HPLC (Waters, Milford MA) on a Waters Xbridge BEH130 Prep C-18 column. The mobile phase comprised gradients of degassed deionized water with 0.1% TFA and acetonitrile with 0.1% TFA, at a flow rate of 21 mL/min. Peptide was detected by a UV detector at 214 nm; fractions with product were collected and lyophilized. The product was confirmed via electrospray ionization mass spectrometry (ESI-MS).

Palmese L.L., Fan M., Scott R.A., Tan H, & Kiick K.L. (2020). Multi-stimuli-responsive, liposome-crosslinked poly(ethylene glycol) hydrogels for drug delivery. Journal of biomaterials science. Polymer edition, 32(5), 635-656.

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