Immunoblots of cell lysates using ERK1/2-specific antibodies were performed using Hybond–P membranes and visualized using ECF substrate (GE Healthcare) according to the manufacturer’s protocol. Fluorescence emission was detected using Typhoon Trio high performance fluorescence scanner (GE Healthcare) and quantified using Image Quant TL software (GE Healthcare). Phospho-array analysis was performed using Proteome Profiler Human Phospho-kinase Array (R&D Systems) as described by the manufacturer. For imaging, blots were incubated with ECL (GE Healthcare) and exposed to ECL Hyperfilm (GE Healthcare). Phospho-kinase array results were quantified by integrating the dot blot densities using Image J software (NIH). Immunoblots from biopsies were analyzed as described24 (link).
Typhoon trio high performance fluorescence scanner
Manufactured by GE Healthcare
The Typhoon Trio is a high-performance fluorescence scanner designed for a variety of applications. It is capable of scanning a wide range of sample types, including gels, blots, and microplates, with high sensitivity and resolution. The scanner utilizes multiple excitation lasers and detection channels to capture fluorescence signals across different wavelength ranges.
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Lab products found in correlation
2 protocols using typhoon trio high performance fluorescence scanner
1
Phospho-kinase Profiling in Cell Lysates
2
Phospho-kinase Profiling in Cell Lysates
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Immunoblots of cell lysates using ERK1/2-specific antibodies were performed using Hybond–P membranes and visualized using ECF substrate (GE Healthcare) according to the manufacturer’s protocol. Fluorescence emission was detected using Typhoon Trio high performance fluorescence scanner (GE Healthcare) and quantified using Image Quant TL software (GE Healthcare). Phospho-array analysis was performed using Proteome Profiler Human Phospho-kinase Array (R&D Systems) as described by the manufacturer. For imaging, blots were incubated with ECL (GE Healthcare) and exposed to ECL Hyperfilm (GE Healthcare). Phospho-kinase array results were quantified by integrating the dot blot densities using Image J software (NIH). Immunoblots from biopsies were analyzed as described24 (link).
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