Detection kits for malondialdehyde

Donepezil hydrochloride tablets (lot: 110822A) were purchased from Eisai China and prepared in a mixed suspension at 0.1 mg/ml. Aβ25‐35 (lot: Y‐0044) was obtained from Bioss Biotech. Streptavidin–biotin complex and terminal deoxynucleotidyl transferase dUTP nick‐end labeling (TUNEL) apoptosis detection kits were purchased from Boster Biotech. A real‐time reverse transcription–quantitative polymerase chain reaction (RT‐qPCR) kit was obtained from TransGen Biotech. Antibodies against Bcl‐2 Bax, and β‐actin were purchased from Bio‐Rad Laboratories. A brain stereotaxic apparatus (ZH‐LAN‐STAR, C type) was obtained from Huaibei Zhenhua Biological Equipment. An inverted microscope (S70) was purchased from Leica. A ChemiDoc XRS gel imaging and analysis system was obtained from Bio‐Rad Laboratories. An SP‐9000 immunohistochemistry kit was purchased from Beijing ZSGB Biological Engineering. PCR amplification instrument (TC‐512) was from Techen. Detection kits for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and ACHE were purchased from Nanjing Jiancheng Bioengineering Research Institute. Rabbit anti‐GFAP antibody (lot: 16825–1‐AP) was obtained from Proteintech. Rabbit anti‐IBA1 antibody (lot: bs‐1363R) was from Bioss Biotech.

Tyrosine was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Anti-MAPK (p38/ERK) antibody and anti-TGF-β antibody were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Porcine primers were designed and synthesized by Generay Biotechnology Co. (Shanghai, China). Trizol reagent and AccuPower GreenStarTM qPCR PreMix kit were purchased from Applied Biosystems (Foster City, CA, USA). ELISA kits of diTyrosine (Dityr), advanced oxidized protein products (AOPPs), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBiL), cross-linked carboxy-terminal telopeptide type I collagen (ICTP), and N-terminal procollagen III propeptide (PIIINP) were obtained from Xiamen Huijia Bioengineering Institute (Xiamen, China). Detection kits for malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPX), superoxide dismutase (SOD), and hematoxylin and eosin (HE) and Masson staining were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other chemicals used in the experiments were of the highest quality commercially available.