Fluoride heparin coated tubes

Manufactured by Sarstedt

Sourced in Germany

Fluoride/heparin-coated tubes are designed for the collection and storage of blood samples. The tubes feature a coating that helps to preserve and stabilize specific analytes in the blood, such as glucose and certain enzymes. These tubes are commonly used in clinical and diagnostic settings to ensure accurate and reliable test results.

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Lab products found in correlation

Ezglu 30k, by Merck Group (2 mentions) Beckman microcentrifuge, by Beckman Coulter (2 mentions) Ascencia meter, by Bayer (2 mentions) Eglp 35k, by Merck Group (1 mentions) Gl 32k, by Merck Group (1 mentions)

2 protocols using fluoride heparin coated tubes

Blood Glucose and Hormone Quantification

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Blood samples were collected from the tail vein of animals into ice-chilled heparin-coated microcentrifuge tubes. Blood glucose was measured using a portable Ascencia meter (Bayer Healthcare, Newbury, Berkshire, UK). For plasma insulin and glucagon, blood was collected in chilled fluoride/heparin-coated tubes (Sarstedt, Numbrecht, Germany) and centrifuged using a Beckman microcentrifuge (Beckman Instruments, Galway, Ireland) for 10 min at 12,000 rpm. Plasma was then stored at – 20 °C. For hormone determination from tissues, samples underwent acid–ethanol extraction (HCl: 1.5% v/v, ethanol: 75% v/v, H₂O:23.5% v/v). Insulin concentrations were subsequently assessed by an in-house radioimmunoassay [34 (link)]. Plasma glucagon and pancreatic glucagon content were measured using glucagon ELISA (EZGLU-30 K, Merck Millipore), or RIA kit (250-tubes GL-32 K, Millipore, USA), respectively.

Sarnobat D., Moffett R.C., Flatt P.R, & Tarasov A.I. (2021). Effects of first-line diabetes therapy with biguanides, sulphonylurea and thiazolidinediones on the differentiation, proliferation and apoptosis of islet cell populations. Journal of Endocrinological Investigation, 45(1), 95-103.

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Analytical Techniques for Metabolic Hormones

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Blood samples were collected from the tail vein of animals into ice-chilled heparin-coated microcentrifuge tubes. Blood glucose was measured using a portable Ascencia meter (Bayer Healthcare, Newbury, Berkshire, UK). For plasma insulin and glucagon, blood was collected in chilled fluoride/heparin-coated tubes (Sarstedt, Numbrecht, Germany) and centrifuged using a Beckman microcentrifuge (Beckman Instruments, Galway, Ireland) for 10 minutes at 12,000 rpm. Plasma was extracted and stored at - 20°C. For hormone determination from tissues, samples underwent acid-ethanol extraction (HCl: 1.5% v/v, ethanol: 75% v/v, H₂O:23.5% v/v). Insulin concentrations were subsequently assessed by an in-house radioimmunoassay [28 (link)]. Plasma glucagon, pancreatic glucagon and GLP-1 content were measured using glucagon ELISA (EZGLU-30K, Merck Millipore), or RIA kit (250-tubes GL-32K, Millipore, USA) and GLP-1 ELISA kit Active (EGLP-35K, Millipore, MA, USA), respectively.

Sarnobat D., Moffett C.R., Tanday N., Reimann F., Gribble F.M., Flatt P.R, & Tarasov A.I. (2020). Antidiabetic drug therapy alleviates type 1 diabetes in mice by promoting pancreatic α-cell transdifferentiation. Biochemical pharmacology, 182, 114216.

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