Anti t bet pe

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-T-bet PE is a laboratory reagent used for the detection and analysis of T-bet, a transcription factor involved in the regulation of T-cell differentiation. It is a fluorescently-labeled antibody that binds specifically to T-bet and can be used in flow cytometry, immunofluorescence, and other applications.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti t bet pe

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunofluorescence staining, dead cells were excluded with the Near-IR Dead-Cell stain (Invitrogen). Antibodies used were: anti-CD3 PE-Cy7 or APC, anti-CD8 PerCP-Cy5.5 or eFluor 450, anti-CD69 FITC (eBioscience); anti-CD161 PE or APC, anti-CD4 VioGreen (Miltenyi Biotec); anti-Vα7.2 PE or FITC or PE-Cy7, anti-CD107α PE-Cy7, anti-Granzyme A PerCP-Cy5.5, anti-Perforin Pacific Blue, anti-granulysin PE, anti-FasL PE (BioLegend); anti-Granzyme B AlexaFluor700, anti-Perforin FITC, anti-Ki67 FITC (BD Biosciences), anti-Granzyme B APC (Invitrogen); anti-Granzyme K FITC (Immunotools); anti-T-bet PE (Santa Cruz Biotechnology); anti-Granzyme A FITC, anti-Blimp1 AlexaFluor488 (R&D); anti-CD8β PE (Beckman Coulter).
Data were collected on the flow cytometers LSRII (BD Biosciences) or MACSQuant (Miltenyi Biotec), and was analyzed using FlowJo v9.6 (TreeStar). For ImageStream analysis, see Supplementary Methods.

Kurioka A., Ussher J.E., Cosgrove C., Clough C., Fergusson J.R., Smith K., Kang Y.H., Walker L.J., Hansen T.H., Willberg C.B, & Klenerman P. (2014). MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets. Mucosal Immunology, 8(2), 429-440.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell suspensions were analysed using BD LSRII research flowcytometry, after staining with combinations of the following antibodies and their isotype controls: Anti-CD4-APC-CY7 (BD Pharmingen, USA), anti CD25-Alexa Fluor 700 (BD Pharmingen, USA), anti CCR4-PE-CY7 (BD Pharmingen, USA), anti CCR6-Alexa Fluor 647 (BioLegend), anti CD127-Alexa Fluor 647 (BD Pharmingen, USA), anti FoxP3-Alexa Fluor 488 (BD Pharmingen, USA), anti GATA-3-Alexa Fluor 647 (BD Pharmingen, USA), and anti Tbet-PE (Santa Cruz). For intracellular staining, cells were fixed and permeabilised using the cytofix/Cytoperm kit (BD Biosciences, USA) as per manufacturer's protocol. Data were analysed using FlowJo ® version 7.6 (Tree Star Inc., USA). Lymphocyte population was selected then identification of pure CD4 + proportion of T cells was gated on isotype control. The cell populations were calculated as percentages of CD4 + T cells to the whole lymphocytic count and presented as mean  SD. Identification of different T cell subsets and gating is shown in Figure S6, S7, S8 and S9.

Kotb I.S., Lewis B.J., Barker R.N, & Ormerod A.D. (2018). Differential effects of phototherapy, adalimumab and betamethasone-calcipotriol on effector and regulatory T cells in psoriasis. The British journal of dermatology, 179(1).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!